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The complex is a multimer of two discrete proteins, P45 and P50, with significant similarity to bacterial dCTP deaminase\/dUTPase and DNA flavoprotein, respectively. When tested in PCR, only recombinant P45 exhibited enhancing activity. P45 was shown to function as a dUTPase, converting dUTP to dUMP and inorganic pyrophosphate.\n            <jats:italic>Pfu<\/jats:italic>\n            dUTPase improves the yield of products amplified with\n            <jats:italic>Pfu<\/jats:italic>\n            DNA polymerase by preventing dUTP incorporation and subsequent inhibition of the polymerase by dU-containing DNA. dUTP was found to accumulate during PCR through dCTP deamination and to limit the efficiency of PCRs carried out with archaeal DNA polymerases. In the absence of dUTP inhibition, the combination of cloned\n            <jats:italic>Pfu<\/jats:italic>\n            DNA polymerase and\n            <jats:italic>Pfu<\/jats:italic>\n            dUTPase (\n            <jats:italic>PfuTurbo<\/jats:italic>\n            DNA polymerase) can amplify longer targets in higher yield than\n            <jats:italic>Taq<\/jats:italic>\n            DNA polymerase.\n            <jats:italic>In vivo<\/jats:italic>\n            , archaeal dUTPases may play an essential role in preventing dUTP incorporation and inhibition of DNA synthesis by family B DNA polymerases.\n          <\/jats:p>","DOI":"10.1073\/pnas.012372799","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:36:44Z","timestamp":1027694204000},"page":"596-601","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":61,"title":["Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation"],"prefix":"10.1073","volume":"99","author":[{"given":"Holly H.","family":"Hogrefe","sequence":"first","affiliation":[{"name":"Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie J.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley R.","family":"Scott","sequence":"additional","affiliation":[{"name":"Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirk B.","family":"Nielson","sequence":"additional","affiliation":[{"name":"Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2002,1,8]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"3546","DOI":"10.1093\/nar\/24.18.3546","volume":"24","author":"Cline J","year":"1996","unstructured":"J Cline, J C Braman, H H Hogrefe Nucleic Acids Res 24, 3546\u20133551 (1996).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1093\/nar\/22.15.3259","volume":"22","author":"Flaman J-M","year":"1994","unstructured":"J-M Flaman, T Frebourg, V Moreau, F Charbonnier, C Martin, C Ishioka, S H Friend, R Iggo Nucleic Acids Res 22, 3259\u20133260 (1994).","journal-title":"Nucleic Acids Res"},{"key":"e_1_3_4_3_2","volume-title":"PCR Primer","author":"Cha R S","year":"1995","unstructured":"R S Cha, W G Thilly PCR Primer, eds C W Dieffenbach, G S Dveksler (Cold Spring Harbor Lab. 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