{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:13:43Z","timestamp":1782360823745,"version":"3.54.5"},"reference-count":33,"publisher":"National Academy of Sciences","issue":"17","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2004,4,27]]},"abstract":"<jats:p>DNA helicases are enzymes capable of unwinding double-stranded DNA (dsDNA) to provide the single-stranded DNA template required in many biological processes. Among these, UvrD, an essential DNA repair enzyme, has been shown to unwind dsDNA while moving 3\u2032-5\u2032 on one strand. Here, we use a single-molecule manipulation technique to monitor real-time changes in extension of a single, stretched, nicked dsDNA substrate as it is unwound by a single enzyme. This technique offers a means for measuring the rate, lifetime, and processivity of the enzymatic complex as a function of ATP, and for estimating the helicase step size. Strikingly, we observe a feature not seen in bulk assays: unwinding is preferentially followed by a slow, enzyme-translocation-limited rezipping of the separated strands rather than by dissociation of the enzymatic complex followed by quick rehybridization of the DNA strands. We address the mechanism underlying this phenomenon and propose a fully characterized model in which UvrD switches strands and translocates backwards on the other strand, allowing the DNA to reanneal in its wake.<\/jats:p>","DOI":"10.1073\/pnas.0306713101","type":"journal-article","created":{"date-parts":[[2004,4,12]],"date-time":"2004-04-12T21:04:03Z","timestamp":1081803843000},"page":"6439-6444","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":170,"title":["Single-molecule assay reveals strand switching and enhanced processivity of UvrD"],"prefix":"10.1073","volume":"101","author":[{"given":"Marie-No\u00eblle","family":"Dessinges","sequence":"first","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, Unit\u00e9 Mixte de Recherche 8550, Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France; Departement de Biologie, Ecole Normale Sup\u00e9rieure, 46 Rue d'Ulm, 75231 Paris Cedex 05, France; and Laboratoire de Biotechnologies et Pharmacologie G\u00e9n\u00e9tique Appliqu\u00e9e, Ecole Normale Sup\u00e9rieure Cachan, Unit\u00e9 Mixte de Recherche 8113, Centre National de la Recherche Scientifique, 61 Avenue du Pr\u00e9sident Wilson, 94235..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timoth\u00e9e","family":"Lionnet","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, Unit\u00e9 Mixte de Recherche 8550, Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France; Departement de Biologie, Ecole Normale Sup\u00e9rieure, 46 Rue d'Ulm, 75231 Paris Cedex 05, France; and Laboratoire de Biotechnologies et Pharmacologie G\u00e9n\u00e9tique Appliqu\u00e9e, Ecole Normale Sup\u00e9rieure Cachan, Unit\u00e9 Mixte de Recherche 8113, Centre National de la Recherche Scientifique, 61 Avenue du Pr\u00e9sident Wilson, 94235..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu Guang","family":"Xi","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, Unit\u00e9 Mixte de Recherche 8550, Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France; Departement de Biologie, Ecole Normale Sup\u00e9rieure, 46 Rue d'Ulm, 75231 Paris Cedex 05, France; and Laboratoire de Biotechnologies et Pharmacologie G\u00e9n\u00e9tique Appliqu\u00e9e, Ecole Normale Sup\u00e9rieure Cachan, Unit\u00e9 Mixte de Recherche 8113, Centre National de la Recherche Scientifique, 61 Avenue du Pr\u00e9sident Wilson, 94235..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bensimon","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, Unit\u00e9 Mixte de Recherche 8550, Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France; Departement de Biologie, Ecole Normale Sup\u00e9rieure, 46 Rue d'Ulm, 75231 Paris Cedex 05, France; and Laboratoire de Biotechnologies et Pharmacologie G\u00e9n\u00e9tique Appliqu\u00e9e, Ecole Normale Sup\u00e9rieure Cachan, Unit\u00e9 Mixte de Recherche 8113, Centre National de la Recherche Scientifique, 61 Avenue du Pr\u00e9sident Wilson, 94235..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Croquette","sequence":"additional","affiliation":[{"name":"Laboratoire de Physique Statistique, Ecole Normale Sup\u00e9rieure, Unit\u00e9 Mixte de Recherche 8550, Centre National de la Recherche Scientifique, 24 Rue Lhomond, 75231 Paris Cedex 05, France; Departement de Biologie, Ecole Normale Sup\u00e9rieure, 46 Rue d'Ulm, 75231 Paris Cedex 05, France; and Laboratoire de Biotechnologies et Pharmacologie G\u00e9n\u00e9tique Appliqu\u00e9e, Ecole Normale Sup\u00e9rieure Cachan, Unit\u00e9 Mixte de Recherche 8113, Centre National de la Recherche Scientifique, 61 Avenue du Pr\u00e9sident Wilson, 94235..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2004,4,12]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583502003852"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583502003864"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063089"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.65.070196.001125"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80088-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01734-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7801120"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.15.9197"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01700.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67506-4"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71522-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.572"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.22.8664"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a135631"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303581200"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(60)91350-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5257.1835"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/35009144"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.22.12002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.8.2519-2526.1999"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3185"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/35053131"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/35053124"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.25.11782"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/1438-5171(200204)3:1<43::AID-SIMO43>3.0.CO;2-S"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132378799"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5298.377"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01276-7"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01585"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01577"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9822269"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(02)01277-9"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00053a028"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0306713101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T09:55:20Z","timestamp":1649757320000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0306713101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,12]]},"references-count":33,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2004,4,27]]}},"alternative-id":["10.1073\/pnas.0306713101"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0306713101","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,12]]},"assertion":[{"value":"2003-10-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-02-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2004-04-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}