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To test whether GLD-3 might stimulate GLD-2 by recruiting it to RNA, we tethered\n                    <jats:italic>C. elegans<\/jats:italic>\n                    GLD-2 to mRNAs in\n                    <jats:italic>Xenopus<\/jats:italic>\n                    oocytes by using MS2 coat protein. Tethered GLD-2 adds poly(A) and stimulates translation of the mRNA, demonstrating that recruitment is sufficient to stimulate polyadenylation activity. We use the same tethered assay to identify human and mouse poly(A) polymerases related to GLD-2. This may provide entrees to previously uncharacterized modes of polyadenylation in mammalian cells.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0400779101","type":"journal-article","created":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T14:58:04Z","timestamp":1080658684000},"page":"4407-4412","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":123,"title":["Mammalian GLD-2 homologs are poly(A) polymerases"],"prefix":"10.1073","volume":"101","author":[{"given":"Jae Eun","family":"Kwak","sequence":"first","affiliation":[{"name":"Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706-1544"}]},{"given":"Liaoteng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706-1544"}]},{"given":"Scott","family":"Ballantyne","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706-1544"}]},{"given":"Judith","family":"Kimble","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706-1544"}]},{"given":"Marvin","family":"Wickens","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706-1544"}]}],"member":"341","published-online":{"date-parts":[[2004,3,30]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"Wickens M. 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