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Indeed, we observed that upon irradiation vector-producing cells underwent apoptotic cell death, monitored by annexin V and propidium iodide staining, and were phagocytosed by DCs. Lymphocytes obtained from a patient affected by a MAGE-3<jats:sup>+<\/jats:sup>melanoma, were stimulated<jats:italic>in vitro<\/jats:italic>with autologous DCs previously exposed to irradiated MAGE-3-expressing cells. This procedure led to the induction of MAGE-3-specific cytotoxic effectors, directed against a yet unknown MAGE-3 epitope presented by HLA-A*B5201 molecules. These data demonstrate that DCs can present engulfed human TAAs, thus providing strategies for cancer vaccination.<\/jats:p>","DOI":"10.1073\/pnas.040540197","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:41:48Z","timestamp":1027694508000},"page":"2185-2190","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":109,"title":["Dendritic cells acquire the MAGE-3 human tumor antigen from apoptotic cells and induce a class I-restricted T cell response"],"prefix":"10.1073","volume":"97","author":[{"given":"Vincenzo","family":"Russo","sequence":"first","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Silvia","family":"Tanzarella","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Piero","family":"Dalerba","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Donata","family":"Rigatti","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Patrizia","family":"Rovere","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Antonello","family":"Villa","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. Raffaele and University of Milan, 20132 Milan, Italy."}]},{"given":"Claudio","family":"Bordignon","sequence":"additional","affiliation":[{"name":"Telethon Institute of Gene Therapy, and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H.S. Raffaele, 20132 Milan, Italy; and Department of Pharmacology and Consiglio Nazionale delle Ricerche, Istituto Scientifico H.S. 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