{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T11:03:14Z","timestamp":1775559794092,"version":"3.50.1"},"reference-count":29,"publisher":"Proceedings of the National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2005,3,22]]},"abstract":"<jats:p>\n            Neural Hu proteins (HuB\/C\/D) are RNA-binding proteins that have been shown to induce neuronal differentiation activity when overexpressed in immature neural progenitor cells or undifferentiated neuronal tumors. Newly generated\n            <jats:italic>HuD-<\/jats:italic>\n            deficient mice exhibited a transient impaired-cranial-nerve-development phenotype at an early embryonic stage. Adult\n            <jats:italic>HuD<\/jats:italic>\n            -deficient mice exhibited an abnormal hind-limb reflex and poor rotarod performance. Analysis of neurosphere formation revealed that the number and self-renewal capacity of the neural stem\/progenitor cells were increased in\n            <jats:italic>HuD<\/jats:italic>\n            -deficient mice.\n            <jats:italic>HuD<\/jats:italic>\n            -deficient primary neurospheres also generated a smaller number of neurons. Cohort analysis of the cellular proliferative activity by using BrdUrd and iododeoxuridine labeling revealed that the number of differentiating quiescent cells in the embryonic cerebral wall was decreased. Long-term administration of BrdUrd revealed that the number of slowly dividing stem cells in the adult subventricular zone was increased in the\n            <jats:italic>HuD<\/jats:italic>\n            -deficient mice. Taken together, the results suggest that HuD is required at multiple points during neuronal development, including negative regulation of proliferative activity and neuronal cell-fate acquisition of neural stem\/progenitor cells.\n          <\/jats:p>","DOI":"10.1073\/pnas.0407523102","type":"journal-article","created":{"date-parts":[[2005,3,12]],"date-time":"2005-03-12T01:24:50Z","timestamp":1110590690000},"page":"4625-4630","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":192,"title":["The RNA-binding protein HuD regulates neuronal cell identity and maturation"],"prefix":"10.1073","volume":"102","author":[{"given":"Wado","family":"Akamatsu","sequence":"first","affiliation":[{"name":"Departments of Physiology, Microbiology, and Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Histology and Neurobiology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan; Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan"}]},{"given":"Hiroaki","family":"Fujihara","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Microbiology, and Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Histology and Neurobiology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan; Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan"}]},{"given":"Takayuki","family":"Mitsuhashi","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Microbiology, and Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Histology and Neurobiology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan; Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan"}]},{"given":"Masato","family":"Yano","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Microbiology, and Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Histology and Neurobiology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan; Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan; and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan"}]},{"given":"Shinsuke","family":"Shibata","sequence":"additional","affiliation":[{"name":"Departments of Physiology, Microbiology, and Pediatrics, Keio University School of Medicine, Tokyo 160-8582, Japan; Department of Histology and Neurobiology, Dokkyo University School of Medicine, Tochigi 321-0293, Japan; Department of Molecular Genetics, Tohoku University School of Medicine, Sendai 980-8575, Japan; 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