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GFP-labeled virus could also be found in the cytoplasm of neighboring cells that were in contact with the projections. In addition, projection formation could be inhibited by using the actin-stabilizing drug jasplakinolide and could be induced by using the Rho kinase inhibitor Y27632. Analyzing the effect of these drugs on intercellular virus spread indicated that the observed US3-induced alterations in the host cytoskeleton are associated with enhanced intercellular virus spread, thereby suggesting a previously undescribed aspect of alphaherpesvirus spread.\n          <\/jats:p>","DOI":"10.1073\/pnas.0409099102","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T00:25:46Z","timestamp":1118449546000},"page":"8990-8995","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":134,"title":["Cytoskeletal rearrangements and cell extensions induced by the US3 kinase of an alphaherpesvirus are associated with enhanced spread"],"prefix":"10.1073","volume":"102","author":[{"given":"Herman W.","family":"Favoreel","sequence":"first","affiliation":[{"name":"Laboratory of Virology and Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium; and Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, 2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geert","family":"Van Minnebruggen","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium; and Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, 2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Adriaensen","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium; and Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, 2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans J.","family":"Nauwynck","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium; and Laboratory of Cell Biology and Histology, Department of Biomedical Sciences, University of Antwerp, 2020 Antwerp, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2005,6,10]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/35074539"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069784"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/378636a0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.5.3904-3915.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9103"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.5.4192-4204.1998"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35060032"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1101-992"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1101509"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.5.1007"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.4.657"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200203150"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404686101"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.061029798"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.1.79-95.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050070090"},{"key":"e_1_3_2_17_2","first-page":"465","volume":"43","year":"1973","unstructured":"Baskerville, A., McFerran, J. 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