{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T03:44:41Z","timestamp":1773459881115,"version":"3.50.1"},"reference-count":18,"publisher":"Proceedings of the National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2003,3,4]]},"abstract":"<jats:p>\n                    V\n                    <jats:sub>o<\/jats:sub>\n                    V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase is responsible for acidification of eukaryotic intracellular compartments and ATP synthesis of Archaea and some eubacteria. From the similarity to F\n                    <jats:sub>o<\/jats:sub>\n                    F\n                    <jats:sub>1<\/jats:sub>\n                    -ATP synthase, V\n                    <jats:sub>o<\/jats:sub>\n                    V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase has been assumed to be a rotary motor, but to date there are no experimental data to support this. Here we visualized the rotation of single molecules of V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase, a catalytic subcomplex of V\n                    <jats:sub>o<\/jats:sub>\n                    V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase. V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase from\n                    <jats:italic>Thermus thermophilus<\/jats:italic>\n                    was immobilized onto a glass surface, and a bead was attached to the D or F subunit through the biotin-streptavidin linkage. In both cases we observed ATP-dependent rotations of beads, the direction of which was always counterclockwise viewed from the membrane side. Given that three ATP molecules are hydrolyzed per one revolution, rates of rotation agree consistently with rates of ATP hydrolysis at saturating ATP concentrations. This study provides experimental evidence that V\n                    <jats:sub>o<\/jats:sub>\n                    V\n                    <jats:sub>1<\/jats:sub>\n                    -ATPase is a rotary motor and that both D and F subunits constitute a rotor shaft.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0436796100","type":"journal-article","created":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T13:36:00Z","timestamp":1046784960000},"page":"2312-2315","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":174,"title":["Evidence for rotation of V\n                    <sub>1<\/sub>\n                    -ATPase"],"prefix":"10.1073","volume":"100","author":[{"given":"Hiromi","family":"Imamura","sequence":"first","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Masahiro","family":"Nakano","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Hiroyuki","family":"Noji","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Eiro","family":"Muneyuki","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Shoji","family":"Ohkuma","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Masasuke","family":"Yoshida","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]},{"given":"Ken","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"ATP System Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation (JST), 5800-3 Nagatsuta, Midori-ku, Yokohama 226-0026, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), JST, Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan; Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan; and Department of Molecular and Cellular..."}]}],"member":"341","published-online":{"date-parts":[[2003,2,21]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm729"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35089509"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(93)90063-L"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.13.1.779"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45830-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a122184"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.31.18843"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/386299a0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00077-3"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32708-4"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.18.13955"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.41.28909"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.42.26787"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0262449"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.32.20504"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241365698"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42351-9"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/35073513"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0436796100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:23:31Z","timestamp":1649773411000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0436796100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,21]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2003,3,4]]}},"alternative-id":["10.1073\/pnas.0436796100"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0436796100","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1012643.186401","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1012643.192827","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,2,21]]},"assertion":[{"value":"2002-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}