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When RNA from\n            <jats:italic>rrp6<\/jats:italic>\n            \u0394 yeast \nis compared with wild-type on whole-genome microarrays, numerous \nintergenic loci exhibit an increased mutant\/wild type signal ratio. \nAmong these loci, we found one encoding a new C\/D box small nucleolar \nRNA, as well as a surprising number that gave rise to heterogeneous \nTrf4p-polyadenylated RNAs with lengths of \u2248250\u2013500 nt. \nThis class of RNAs is not easily detected in wild-type cells and \nappears associated with promoters. Fine mapping of several such \ntranscripts shows they originate near known promoter elements but do \nnot usually extend far enough to act as mRNAs, and may regulate the \ntranscription of downstream mRNAs. Rather than being uninformative \ntranscriptional \u201cnoise,\u201d we hypothesize that these \ntranscripts reflect important features of RNA polymerase activity at \nthe promoter. This activity is normally undetectable in wild-type \ncells because the transcripts are somehow distinguished from true \nmRNAs and are degraded in an Rrp6p-dependent fashion in the \nnucleus.\n          <\/jats:p>","DOI":"10.1073\/pnas.0507783103","type":"journal-article","created":{"date-parts":[[2006,2,17]],"date-time":"2006-02-17T01:43:49Z","timestamp":1140140629000},"page":"3262-3267","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":197,"title":["Accumulation of unstable promoter-associated transcripts upon loss of the nuclear exosome subunit Rrp6p in\n            <i>Saccharomyces<\/i>\n            <i>cerevisiae<\/i>"],"prefix":"10.1073","volume":"103","author":[{"given":"Carrie Anne","family":"Davis","sequence":"first","affiliation":[{"name":"Center for Molecular Biology of RNA, Department of Molecular, Cell, and Developmental Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064"}]},{"suffix":"Jr.","given":"Manuel","family":"Ares","sequence":"additional","affiliation":[{"name":"Center for Molecular Biology of RNA, Department of Molecular, Cell, and Developmental Biology, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064"}]}],"member":"341","published-online":{"date-parts":[[2006,2,16]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0092-8674(00)81845-0","volume":"88","author":"Velculescu V. 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