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The predicted protein product of\n                    <jats:italic>tadA<\/jats:italic>\n                    ,\n a recently discovered gene required for tenacious adherence of\n                    <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>\n                    , also has\n significant sequence similarity to members of this superfamily and to\n several unclassified and uncharacterized gene products of both Archaea\n and Bacteria. To understand the relationship of\n                    <jats:italic>tadA<\/jats:italic>\n                    and\n                    <jats:italic>tadA<\/jats:italic>\n                    -like genes to those encoding the putative NTPases\n of type II\/IV secretion, we used a phylogenetic approach to obtain a\n genealogy of 148 NTPase genes and reconstruct a scenario of gene\n superfamily evolution. In this phylogeny, clear distinctions can be\n made between type II and type IV families and their constituent\n subfamilies. In addition, the subgroup containing\n                    <jats:italic>tadA<\/jats:italic>\n                    constitutes a novel and extremely widespread subfamily of the family\n encompassing all putative NTPases of type IV secretion systems. We\n report diagnostic amino acid residue positions for each major\n monophyletic family and subfamily in the phylogenetic tree, and we\n propose an easy method for precisely classifying and naming putative\n NTPase genes based on phylogeny. This molecular key-based method can be\n applied to other gene superfamilies and represents a valuable tool for\n genome analysis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.051436598","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"2503-2508","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":176,"title":["Phylogeny of genes for secretion NTPases: Identification of the widespread\n                    <i>tadA<\/i>\n                    subfamily and development of a diagnostic key for gene classification"],"prefix":"10.1073","volume":"98","author":[{"given":"Paul J.","family":"Planet","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Physicians and\r Surgeons, Columbia University, New York, NY 10032; and\r Molecular Laboratories, American Museum of Natural\r History, New York, NY 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