{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:27:00Z","timestamp":1779344820166,"version":"3.51.4"},"reference-count":42,"publisher":"Proceedings of the National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,3,5]]},"abstract":"<jats:p>\n            Mot1 is an essential yeast Snf2\/Swi2-related ATPase that exerts both positive and negative effects on gene expression.\n            <jats:italic>In vitro<\/jats:italic>\n            , Mot1 can disrupt TATA-binding protein\u2013DNA complexes in an ATP-dependent reaction. This activity can explain Mot1-mediated transcriptional repression, but how Mot1 activates transcription is unknown. We demonstrate that, remarkably, Mot1 is localized\n            <jats:italic>in vivo<\/jats:italic>\n            to promoters for both Mot1-repressed and Mot1-activated genes. Moreover, Mot1 ATPase activity is required for both activation and repression of gene activity. These findings suggest a novel function for the Mot1 ATPase at activated genes, perhaps involving ATP-driven reorganization of the preinitiation complex. Mot1 regulates the expression of \u22483% of yeast genes in cells grown in rich medium. Most of these genes are repressed by Mot1, consistent with Mot1's ATP-dependent TATA-binding protein\u2013DNA dissociating activity. Additionally, \u224877% of the Mot1-repressed genes are involved in the diauxic shift, stress response, mating, or sporulation. The gene sets controlled by NC2 and Srb10 are strongly correlated with the Mot1-controlled set, suggesting that these factors cooperate in transcriptional control on a global scale.\n          <\/jats:p>","DOI":"10.1073\/pnas.052397899","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:37:36Z","timestamp":1027694256000},"page":"2666-2671","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms"],"prefix":"10.1073","volume":"99","author":[{"given":"Arindam","family":"Dasgupta","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226, PO Box 12233, MD2\u201304, Research Triangle Park, NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell P.","family":"Darst","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226, PO Box 12233, MD2\u201304, Research Triangle Park, NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karla J.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226, PO Box 12233, MD2\u201304, Research Triangle Park, NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia A.","family":"Afshari","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226, PO Box 12233, MD2\u201304, Research Triangle Park, NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David T.","family":"Auble","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733; and National Institute of Environmental Health Sciences Microarray Center, 111 Alexander Drive, Building 101, Room D226, PO Box 12233, MD2\u201304, Research Triangle Park, NC 27709"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2002,3,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.831000"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81533-0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.10.1398"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.12.4.1879-1892.1992"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02451793"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.4.2057"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.16.1920"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.1.287"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.12.6668"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.4.2835"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/35015104"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80522-X"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.3.1234"},{"key":"e_1_3_3_14_2","unstructured":"Sandmeier J. 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