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Bacteria devoid of the SeqA protein, which preferentially binds hemimethylated DNA, were found to have a transcriptional profile almost identical to WT bacteria overexpressing Dam methyltransferase. The latter two strains differed from WT in two ways. First, the origin proximal genes were transcribed with increased frequency due to increased gene dosage. Second, chromosomal domains of high transcriptional activity alternate with regions of low activity, and our results indicate that the activity in each domain is modulated in the same way by SeqA deficiency or Dam overproduction. We suggest that the methylation status of the cell is an important factor in forming and\/or maintaining chromosome structure.\n          <\/jats:p>","DOI":"10.1073\/pnas.0538053100","type":"journal-article","created":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T22:56:03Z","timestamp":1050447363000},"page":"4672-4677","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":125,"title":["Role of SeqA and Dam in\n            <i>Escherichia coli<\/i>\n            gene expression: A global\/microarray analysis"],"prefix":"10.1073","volume":"100","author":[{"given":"Anders","family":"L\u00f8bner-Olesen","sequence":"first","affiliation":[{"name":"Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde, Denmark; Biocentrum, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark; and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655"}]},{"given":"Martin G.","family":"Marinus","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde, Denmark; Biocentrum, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark; and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655"}]},{"given":"Flemming G.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Life Sciences and Chemistry, Roskilde University, DK-4000 Roskilde, Denmark; Biocentrum, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark; and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655"}]}],"member":"341","published-online":{"date-parts":[[2003,4,7]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34217-5"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.22.6542"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90283-1"},{"key":"e_1_3_2_4_2","first-page":"782","volume-title":"Escherichia coli and Salmonella: Cellular and Molecular Biology","author":"Marinus M G","year":"1996","unstructured":"M G Marinus Escherichia coli and Salmonella: Cellular and Molecular Biology, eds F C Neidhardt, R Curtiss, J L Ingraham, E C C Lin, K Brooks Low, B Magasanik, W Reznikoff, M Riley, M Schaechter, H E Umbarger (Am. 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