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When introduced into the genome of\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    by\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    -mediated transformation,\n double-stranded RNA-expressing constructs corresponding to four genes,\n                    <jats:italic>AGAMOUS<\/jats:italic>\n                    (\n                    <jats:italic>AG<\/jats:italic>\n                    ),\n                    <jats:italic>CLAVATA3<\/jats:italic>\n                    ,\n                    <jats:italic>APETALA1<\/jats:italic>\n                    , and\n                    <jats:italic>PERIANTHIA<\/jats:italic>\n                    , caused specific\n and heritable genetic interference. The severity of phenotypes varied\n between transgenic lines.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization revealed\n a correlation between a declining\n                    <jats:italic>AG<\/jats:italic>\n                    mRNA accumulation\n and increasingly severe phenotypes in\n                    <jats:italic>AG<\/jats:italic>\n                    (\n                    <jats:italic>RNAi<\/jats:italic>\n                    ) mutants, suggesting that endogenous\n mRNA is the target of double-stranded RNA-mediated genetic\n interference. The ability to generate stably heritable RNAi and the\n resultant specific phenotypes allows us to selectively reduce gene\n function in\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.060034297","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:41:48Z","timestamp":1027680108000},"page":"4985-4990","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":505,"title":["Specific and heritable genetic interference by double-stranded RNA in\n                    <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1073","volume":"97","author":[{"given":"Chiou-Fen","family":"Chuang","sequence":"first","affiliation":[{"name":"Division of Biology 156\u201329, California Institute of Technology,\r Pasadena, CA 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elliot 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