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Consequently, we identified 11,575 TSSs associated with 3,638 annotated genomic features, including 3,599 ORFs, to suggest that most yeast genes have two or more TSSs. In addition, we identified 45 previously undescribed introns, including those affecting current ORF annotations and those spliced alternatively. Furthermore, the analysis revealed 667 transcription units in the intergenic regions and transcripts derived from antisense strands of 367 known features. We also found that 348 ORFs carry TSSs in their 3\u2032-halves to generate sense transcripts starting from inside the ORFs. These results indicate that the budding yeast transcriptome is considerably more complex than previously thought, and it shares many recently revealed characteristics with the transcriptomes of mammals and other higher eukaryotes. Thus, the genome-wide active transcription that generates novel classes of transcripts appears to be an intrinsic feature of the eukaryotic cells. The budding yeast will serve as a versatile model for the studies on these aspects of transcriptome, and the full-length cDNA clones can function as an invaluable resource in such studies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0605645103","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T20:38:56Z","timestamp":1163450336000},"page":"17846-17851","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":198,"title":["A large-scale full-length cDNA analysis to explore the budding yeast transcriptome"],"prefix":"10.1073","volume":"103","author":[{"given":"Fumihito","family":"Miura","sequence":"first","affiliation":[{"name":"*Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa 277-8561, Japan;"},{"name":"Institute for Bioinformatics Research and Development, Japan Science and Technology Agency, Tokyo 102-0081, 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