{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T03:37:43Z","timestamp":1770781063986,"version":"3.50.0"},"reference-count":41,"publisher":"Proceedings of the National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,3,27]]},"abstract":"<jats:p>\n            <jats:italic>Caenorhabditis elegans sqv<\/jats:italic>\n            mutants\n are defective in vulval epithelial invagination and have a severe\n reduction in hermaphrodite fertility. The gene\n            <jats:italic>sqv-7<\/jats:italic>\n            encodes a multitransmembrane hydrophobic protein resembling nucleotide\n sugar transporters of the Golgi membrane. A Golgi vesicle enriched\n fraction of\n            <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n            expressing SQV-7\n transported UDP-glucuronic acid,\n UDP-\n            <jats:italic>N<\/jats:italic>\n            -acetylgalactosamine, and UDP-galactose (Gal) in a\n temperature-dependent and saturable manner. These nucleotide sugars are\n competitive, alternate, noncooperative substrates. The two mutant\n            <jats:italic>sqv-7<\/jats:italic>\n            missense alleles resulted in a severe reduction of\n these three transport activities. SQV-7 did not transport CMP-sialic\n acid, GDP-fucose, UDP-\n            <jats:italic>N<\/jats:italic>\n            -acetylglucosamine, UDP-glucose,\n or GDP-mannose. SQV-7 is able to transport UDP-Gal\n            <jats:italic>in\n vivo<\/jats:italic>\n            , as shown by its ability to complement the phenotype of\n Madin-Darby canine kidney ricin resistant cells, a mammalian cell line\n deficient in UDP-Gal transport into the Golgi. These results\n demonstrate that unlike most nucleotide sugar transporters, SQV-7 can\n transport multiple distinct nucleotide sugars. We propose that SQV-7\n translocates multiple nucleotide sugars into the Golgi lumen for\n the biosynthesis of glycoconjugates that play a pivotal role\n in development.\n          <\/jats:p>","DOI":"10.1073\/pnas.061593098","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:36:44Z","timestamp":1027694204000},"page":"3738-3743","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":107,"title":["SQV-7, a protein involved in\n            <i>Caenorhabditis elegans<\/i>\n            epithelial invagination and early embryogenesis, transports UDP-glucuronic acid, UDP-\n            <i>N<\/i>\n            - acetylgalactosamine, and UDP-galactose"],"prefix":"10.1073","volume":"98","author":[{"given":"Patricia","family":"Berninsone","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University School\r of Dental Medicine, Boston, MA 02118; and Howard Hughes\r Medical Institute, Department of Biology, Massachusetts Institute of\r Technology, Cambridge, MA 02139"}]},{"given":"Ho-Yon","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University School\r of Dental Medicine, Boston, MA 02118; and Howard Hughes\r Medical Institute, Department of Biology, Massachusetts Institute of\r Technology, Cambridge, MA 02139"}]},{"given":"Irina","family":"Zemtseva","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University School\r of Dental Medicine, Boston, MA 02118; and Howard Hughes\r Medical Institute, Department of Biology, Massachusetts Institute of\r Technology, Cambridge, MA 02139"}]},{"given":"H. Robert","family":"Horvitz","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University School\r of Dental Medicine, Boston, MA 02118; and Howard Hughes\r Medical Institute, Department of Biology, Massachusetts Institute of\r Technology, Cambridge, MA 02139"}]},{"given":"Carlos B.","family":"Hirschberg","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Boston University School\r of Dental Medicine, Boston, MA 02118; and Howard Hughes\r Medical Institute, Department of Biology, Massachusetts Institute of\r Technology, Cambridge, MA 02139"}]}],"member":"341","published-online":{"date-parts":[[2001,3,20]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5781(06)80022-8"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(96)80036-4"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-215-44121"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.2.728"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06480.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.18.3565"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.13.2623"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/22336"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/22343"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.41.26265"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.49"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37590-2"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3897"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.15.8851"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42335-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.25986"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.3.968"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.3.974"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.33.22915"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26165"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.7888"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.19.12616"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi992363l"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10707"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.18.12665"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35596"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.7.4474"},{"key":"e_1_3_3_28_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, E F Fritsch, T Maniatis Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. 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