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In this study, we present evidence for a previously uncharacterized poly(A) recognition motif consisting of tandem CCCH zinc fingers. We have probed the nucleic acid binding properties of a yeast protein, Nab2, that contains this zinc finger motif. Results of this study reveal that the seven tandem CCCH zinc fingers of Nab2 specifically bind to polyadenosine RNA with high affinity. Furthermore, we demonstrate that a human protein, ZC3H14, which contains CCCH zinc fingers homologous to those found in Nab2, also specifically binds polyadenosine RNA. Thus, we propose that these proteins are members of an evolutionarily conserved family of poly(A) RNA binding proteins that recognize poly(A) RNA through a fundamentally different mechanism than previously characterized RNA recognition motif-containing poly(A) binding proteins.<\/jats:p>","DOI":"10.1073\/pnas.0701244104","type":"journal-article","created":{"date-parts":[[2007,7,13]],"date-time":"2007-07-13T21:00:59Z","timestamp":1184360459000},"page":"12306-12311","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":80,"title":["Recognition of polyadenosine RNA by zinc finger proteins"],"prefix":"10.1073","volume":"104","author":[{"given":"Seth M.","family":"Kelly","sequence":"first","affiliation":[{"name":"Departments of *Biochemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzette A.","family":"Pabit","sequence":"additional","affiliation":[{"name":"Department of Physics, Emory University, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad M.","family":"Kitchen","sequence":"additional","affiliation":[{"name":"Pharmacology, Emory University School of Medicine, Atlanta, GA 30322; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Physics, Emory University, Atlanta, GA 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita A.","family":"Marfatia","sequence":"additional","affiliation":[{"name":"Departments of *Biochemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. 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