{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T06:31:49Z","timestamp":1772692309825,"version":"3.50.1"},"reference-count":43,"publisher":"Proceedings of the National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,2,19]]},"abstract":"<jats:p>\n            Estrogen signaling is mediated by estrogen receptors \u03b1 (ER\u03b1) and \u03b2 (ER\u03b2). Although a consensus has now been reached concerning many physiological functions of ER\u03b1, those of ER\u03b2 are still controversial: When housed and examined in two distant laboratories, mice originating from the same initial ER\u03b2 mutant exhibited widely different phenotypes, which were themselves different from the phenotype of another ER\u03b2 mutant previously generated in our laboratory. Because, in addition to a knockout insertion in exon 3, all these mouse mutants displayed alternative splicing transcripts, we have now constructed a ER\u03b2 mouse mutant (ER\u03b2\n            <jats:sub arrange=\"stagger\">ST<\/jats:sub>\n            <jats:sup arrange=\"stagger\">L\u2212\/L\u2212<\/jats:sup>\n            ) in which exon 3 was cleanly deleted by Cre\/LoxP-mediated excision and was devoid of any transcript downstream of exon 3. Both females and males were sterile. The histology of the ovary was mildly affected, and no histological defects were detected in other organs, neither in females nor in males. Our present results, which are in contrast with previously published data, suggest that, with the notable exception of male and female reproduction, ER\u03b2 is not required in the mouse for the development and homeostasis of the major body systems.\n          <\/jats:p>","DOI":"10.1073\/pnas.0712029105","type":"journal-article","created":{"date-parts":[[2008,2,12]],"date-time":"2008-02-12T02:40:28Z","timestamp":1202784028000},"page":"2433-2438","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":189,"title":["Sterility and absence of histopathological defects in nonreproductive organs of a mouse ER\u03b2-null mutant"],"prefix":"10.1073","volume":"105","author":[{"given":"Maria Cristina","family":"Antal","sequence":"first","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Coll\u00e8ge de France, Universit\u00e9 Louis Pasteur de Strasbourg, 67404 Illkirch Cedex, France"}]},{"given":"Andr\u00e9e","family":"Krust","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Coll\u00e8ge de France, Universit\u00e9 Louis Pasteur de Strasbourg, 67404 Illkirch Cedex, France"}]},{"given":"Pierre","family":"Chambon","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Coll\u00e8ge de France, Universit\u00e9 Louis Pasteur de Strasbourg, 67404 Illkirch Cedex, France"}]},{"given":"Manuel","family":"Mark","sequence":"additional","affiliation":[{"name":"Institut de G\u00e9n\u00e9tique et de Biologie Mol\u00e9culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Coll\u00e8ge de France, Universit\u00e9 Louis Pasteur de Strasbourg, 67404 Illkirch Cedex, France"}]}],"member":"341","published-online":{"date-parts":[[2008,2,19]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.20.3.0370"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4892(01)00105-9"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(03)00348-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.040403.115914"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.7889"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/320134a0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.5925"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00782-X"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend.11.3.9902"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.043497"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.2004-0027"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/me.2005-0459"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15677"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.19.4277"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.143.5.8772"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403041101"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.041617498"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242735799"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111150898"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0731830100"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405571101"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.23.8542-8552.2003"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602194103"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511271103"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703410104"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608951103"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000300303"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.3.680"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1677\/erc.0.0090001"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192561299"},{"key":"e_1_3_3_31_2","first-page":"389","volume-title":"Pathology of the Mouse","author":"Radovsky A","year":"1999","unstructured":"A Radovsky, K Mitsumori, RC Chapin Pathology of the Mouse, eds RR Maronpot, GA Boorman, B Gaul (Cache River, Vienna), pp. 389 (1999)."},{"key":"e_1_3_3_32_2","first-page":"184","volume-title":"International Classification of Rodent Tumours. The Mouse","author":"Rehm S","year":"2001","unstructured":"S Rehm, et al. International Classification of Rodent Tumours. The Mouse, ed U Mohr (Springer, Heidelberg), pp. 184\u2013185 (2001)."},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00089-8"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.191235898"},{"key":"e_1_3_3_35_2","first-page":"450","volume-title":"Pathology of the Mouse","author":"Radovsky A","year":"1999","unstructured":"A Radovsky, JF Mahler Pathology of the Mouse, eds RR Maronpot, GA Boorman, B Gaul (Cache River, Vienna), pp. 450 (1999)."},{"key":"e_1_3_3_36_2","first-page":"120","volume-title":"Histotechnology a Self-Instructional Text","author":"Carson FL","year":"1996","unstructured":"FL Carson Histotechnology a Self-Instructional Text (Am Soc Clin Pathol, Chicago), pp. 120 (1996)."},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2005.05.006"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2007.03.030"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2003.09.002"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.04703.x"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12887"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500752102"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(00)00105-9"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0712029105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T20:24:14Z","timestamp":1649795054000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0712029105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2,19]]},"references-count":43,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2008,2,19]]}},"alternative-id":["10.1073\/pnas.0712029105"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0712029105","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2,19]]},"assertion":[{"value":"2007-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}