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Of the \u224870,000 combinations, we found 37 protein\u2013protein interactions, including 28 that were previously unknown. In some cases, e.g., late transcription factors, both proteins were known to have related roles although there was no prior evidence of physical associations. For some other interactions, neither protein had a known role. In the majority of cases, however, one of the interacting proteins was known to be involved in DNA replication, transcription, virion structure, or host evasion, thereby providing a clue to the role of the other uncharacterized protein in a specific process.<\/jats:p>","DOI":"10.1073\/pnas.080078197","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:41:48Z","timestamp":1027694508000},"page":"4879-4884","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":229,"title":["Genome-wide analysis of vaccinia virus protein\u2013protein interactions"],"prefix":"10.1073","volume":"97","author":[{"given":"Stephen","family":"McCraith","sequence":"first","affiliation":[{"name":"Departments of Genetics and Medicine, Box 357360, Locke Computer Center, and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195; and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0445"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ted","family":"Holtzman","sequence":"additional","affiliation":[{"name":"Departments of Genetics and Medicine, Box 357360, Locke Computer Center, and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195; and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0445"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Moss","sequence":"additional","affiliation":[{"name":"Departments of Genetics and Medicine, Box 357360, Locke Computer Center, and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195; and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0445"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley","family":"Fields","sequence":"additional","affiliation":[{"name":"Departments of Genetics and Medicine, Box 357360, Locke Computer Center, and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195; and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0445"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2000,4,18]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"2637","volume-title":"Fields Virology","author":"Moss B","year":"1996","unstructured":"B Moss Fields Virology, eds N Fields, D M Knipe, P M Howley (Lippincott-Raven, Philadelphia) 2, 2637\u20132671 (1996)."},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11341"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(90)90294-2"},{"key":"e_1_3_2_4_2","first-page":"185","volume-title":"Transcription Mechanisms and Regulation","author":"Moss B","year":"1993","unstructured":"B Moss Transcription Mechanisms and Regulation, eds R Conaway, J Conaway (Raven, New York), pp. 185\u2013205 (1993)."},{"key":"e_1_3_2_5_2","first-page":"775","volume-title":"DNA Replication in Eukaryotic Cells","author":"Traktman P","year":"1996","unstructured":"P Traktman DNA Replication in Eukaryotic Cells, ed M L DePamphilis (Cold Spring Harbor Lab. 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