{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:16:45Z","timestamp":1779113805910,"version":"3.51.4"},"reference-count":43,"publisher":"Proceedings of the National Academy of Sciences","issue":"30","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,7,29]]},"abstract":"<jats:p>\n                    The tumor suppressor p53 is mutationally inactivated in \u224850% of human cancers. Approximately one-third of the mutations lower the melting temperature of the protein, leading to its rapid denaturation. Small molecules that bind to those mutants and stabilize them could be effective anticancer drugs. The mutation Y220C, which occurs in \u224875,000 new cancer cases per annum, creates a surface cavity that destabilizes the protein by 4 kcal\/mol, at a site that is not functional. We have designed a series of binding molecules from an\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis of the crystal structure using virtual screening and rational drug design. One of them, a carbazole derivative (PhiKan083), binds to the cavity with a dissociation constant of \u2248150 \u03bcM. It raises the melting temperature of the mutant and slows down its rate of denaturation. We have solved the crystal structure of the protein\u2013PhiKan083 complex at 1.5-\u212b resolution. The structure implicates key interactions between the protein and ligand and conformational changes that occur on binding, which will provide a basis for lead optimization. The Y220C mutant is an excellent \u201cdruggable\u201d target for developing and testing novel anticancer drugs based on protein stabilization. We point out some general principles in relationships between binding constants, raising of melting temperatures, and increase of protein half-lives by stabilizing ligands.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0805326105","type":"journal-article","created":{"date-parts":[[2008,7,23]],"date-time":"2008-07-23T21:01:25Z","timestamp":1216846885000},"page":"10360-10365","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":339,"title":["Targeted rescue of a destabilized mutant of p53 by an\n                    <i>in silico<\/i>\n                    screened drug"],"prefix":"10.1073","volume":"105","author":[{"given":"Frank M.","family":"Boeckler","sequence":"first","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]},{"given":"Andreas C.","family":"Joerger","sequence":"additional","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]},{"given":"Gaurav","family":"Jaggi","sequence":"additional","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]},{"given":"Trevor J.","family":"Rutherford","sequence":"additional","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]},{"given":"Dmitry B.","family":"Veprintsev","sequence":"additional","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]},{"given":"Alan R.","family":"Fersht","sequence":"additional","affiliation":[{"name":"Centre for Protein Engineering, Medical Research Council Centre, Hills Road, Cambridge CB2 0QH, United Kingdom"}]}],"member":"341","published-online":{"date-parts":[[2008,7,29]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/35042675"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc864"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.060806.091238"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.10081"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210291"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.26.14338"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604209200"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302458200"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203434"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205362"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241629998"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5449.2507"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1835733100"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5751-3-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32481"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0607286103"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20495"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.14675"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309732200"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ci049714"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(00)00129-0"},{"key":"e_1_3_3_22_2","unstructured":"MOE.  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