{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:23:15Z","timestamp":1772137395074,"version":"3.50.1"},"reference-count":63,"publisher":"Proceedings of the National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,4,16]]},"abstract":"<jats:p>\n                    Packaging of cyclophilin A (CypA) into HIV-1 virions is essential for efficient replication; however, the reason for this is unknown. Incorporation is mediated through binding to the Gly-89\u2013Pro-90 peptide bond of the N-terminal domain of HIV-1 capsid (CA\n                    <jats:sup>N<\/jats:sup>\n                    ). Despite the fact that CypA is a peptidyl-prolyl cis\/trans isomerase, catalytic activity on CA\n                    <jats:sup>N<\/jats:sup>\n                    has not been observed previously. We show here, using NMR exchange spectroscopy, that CypA does not only bind to CA\n                    <jats:sup>N<\/jats:sup>\n                    but also catalyzes efficiently the cis\/trans isomerization of the Gly-89\u2013Pro-90 peptide bond. In addition, conformational changes in CA\n                    <jats:sup>N<\/jats:sup>\n                    distal to the CypA binding loop are observed on CypA binding and catalysis. The results provide experimental evidence for efficient CypA catalysis on a natively folded and biologically relevant protein substrate.\n                  <\/jats:p>","DOI":"10.1073\/pnas.082100499","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"5247-5252","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":186,"title":["Catalysis of cis\/trans isomerization in native HIV-1 capsid by human cyclophilin A"],"prefix":"10.1073","volume":"99","author":[{"given":"Daryl A.","family":"Bosco","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Brandeis University, Waltham, MA 02454"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elan Z.","family":"Eisenmesser","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Brandeis University, Waltham, MA 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