{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:22:21Z","timestamp":1781108541506,"version":"3.54.1"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,5,9]]},"abstract":"<jats:p>\n                    hDlg, the human homologue of the\n                    <jats:italic>Drosophila D<\/jats:italic>\n                    iscs-\n                    <jats:italic>l<\/jats:italic>\n                    ar\n                    <jats:italic>g<\/jats:italic>\n                    e (Dlg) tumor suppressor protein, is known to interact with the tumor suppressor protein APC and the human papillomavirus E6 transforming protein. In a two-hybrid screen, we identified a 322-aa serine\/threonine kinase that binds to the PDZ2 domain of hDlg. The mRNA for this PDZ-binding kinase, or PBK, is most abundant in placenta and absent from adult brain tissue. The protein sequence of PBK has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T\/SXV motif.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , PBK binds specifically to PDZ2 of hDlg through its C-terminal T\/SXV motif. PBK and hDlg are phosphorylated at mitosis in HeLa cells, and the mitotic phosphorylation of PBK is required for its kinase activity.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , cdc2\/cyclin B phosphorylates PBK. This evidence shows how PBK could link hDlg or other PDZ-containing proteins to signal transduction pathways regulating the cell cycle or cellular proliferation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.090102397","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"5167-5172","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":186,"title":["Characterization of PDZ-binding kinase, a mitotic kinase"],"prefix":"10.1073","volume":"97","author":[{"given":"Suzanne","family":"Gaudet","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Branton","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Lue","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,4,25]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.9818"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.136.3.669"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2443.1997.1310329.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.18.11943"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90245-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80284-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7834"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.6.949"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37012-6"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(98)00164-7"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65691-X"},{"key":"e_1_3_3_12_2","first-page":"45","volume":"151","author":"Kimura Y","year":"1997","unstructured":"Y Kimura, H Shiozaki, M Hirao, Y Maeno, Y Doki, M Inoue, T Monden, Y Ando-Akatsuka, M Furuse, S Tsukita, et al. 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