{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T04:37:14Z","timestamp":1771043834380,"version":"3.50.1"},"reference-count":44,"publisher":"Proceedings of the National Academy of Sciences","issue":"31","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,8,4]]},"abstract":"<jats:p>Despite the importance of the aberrant polymerization of A\u03b2 in the early pathogenic cascade of Alzheimer's disease, little is known about the induction of A\u03b2 aggregation in vivo. Here we show that induction of cerebral \u03b2-amyloidosis can be achieved in many different brain areas of APP23 transgenic mice through the injection of dilute A\u03b2-containing brain extracts. Once the amyloidogenic process has been exogenously induced, the nature of the induced A\u03b2-deposition is determined by the brain region of the host. Because these observations are reminiscent of a prion-like mechanism, we then investigated whether cerebral \u03b2-amyloidosis also can be induced by peripheral and systemic inoculations or by the intracerebral implantation of stainless steel wires previously coated with minute amounts of A\u03b2-containing brain extract. Results reveal that oral, intravenous, intraocular, and intranasal inoculations yielded no detectable induction of cerebral \u03b2-amyloidosis in APP23 transgenic mice. In contrast, transmission of cerebral \u03b2-amyloidosis through the A\u03b2-contaminated steel wires was demonstrated. Notably, plasma sterilization, but not boiling of the wires before implantation, prevented the induction of \u03b2-amyloidosis. Our results suggest that minute amounts of A\u03b2-containing brain material in direct contact with the CNS can induce cerebral \u03b2-amyloidosis, but that systemic cellular mechanisms of prion uptake and transport to the CNS may not apply to A\u03b2.<\/jats:p>","DOI":"10.1073\/pnas.0903200106","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T01:07:45Z","timestamp":1248224865000},"page":"12926-12931","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":240,"title":["Induction of cerebral \u03b2-amyloidosis: Intracerebral versus systemic A\u03b2 inoculation"],"prefix":"10.1073","volume":"106","author":[{"given":"Yvonne S.","family":"Eisele","sequence":"first","affiliation":[{"name":"Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany;"},{"name":"Graduate School for Cellular and Molecular Neuroscience, University of T\u00fcbingen, D-72074 T\u00fcbingen, Germany;"}]},{"given":"Tristan","family":"Bolmont","sequence":"additional","affiliation":[{"name":"Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany;"}]},{"given":"Mathias","family":"Heikenwalder","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute for Neuropathology, University Hospital, Z\u00fcrich, CH-8091 Switzerland;"}]},{"given":"Franziska","family":"Langer","sequence":"additional","affiliation":[{"name":"Department of Cellular Neurology, Hertie Institute for Clinical Brain Research, University of T\u00fcbingen, D-72076 T\u00fcbingen, Germany;"},{"name":"Graduate School for Cellular and Molecular Neuroscience, University of T\u00fcbingen, D-72074 T\u00fcbingen, Germany;"}]},{"given":"Laura H.","family":"Jacobson","sequence":"additional","affiliation":[{"name":"Nervous System Research, Novartis Institutes for Biomedical Research, CH-4002 Basel, Switzerland;"}]},{"given":"Zheng-Xin","family":"Yan","sequence":"additional","affiliation":[{"name":"SMP GmbH, D-72072 T\u00fcbingen, Germany;"}]},{"given":"Klaus","family":"Roth","sequence":"additional","affiliation":[{"name":"SMP GmbH, D-72072 T\u00fcbingen, Germany;"}]},{"given":"Adriano","family":"Aguzzi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Institute for Neuropathology, University Hospital, Z\u00fcrich, CH-8091 Switzerland;"}]},{"given":"Matthias","family":"Staufenbiel","sequence":"additional","affiliation":[{"name":"Nervous System Research, Novartis Institutes for Biomedical Research, CH-4002 Basel, Switzerland;"}]},{"given":"Lary C.","family":"Walker","sequence":"additional","affiliation":[{"name":"Yerkes National Primate Research Center and Department of Neurology, Emory University, Atlanta, GA 30322; and"}]},{"given":"Mathias","family":"Jucker","sequence":"additional","affiliation":[{"name":"Department of Cellular 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