{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:33:41Z","timestamp":1781109221214,"version":"3.54.1"},"reference-count":31,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,5,9]]},"abstract":"<jats:p>\n                    We previously have shown that DNA demethylation by chicken embryo 5-methylcytosine DNA glycosylase (5-MCDG) needs both RNA and proteins. One of these proteins is a RNA helicase. Further peptides were sequenced, and three of them are identical to the mammalian G\/T mismatch DNA glycosylase. A 3,233-bp cDNA coding for the chicken homologue of human G\/T mismatch DNA glycosylase was isolated and sequenced. The derived amino acid sequence (408 aa) shows 80% identity with the human G\/T mismatch DNA glycosylase, and both the C and N-terminal parts have about 50% identity. As for the highly purified chicken embryo DNA demethylation complex the recombinant protein expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    has both G\/T mismatch and 5-MCDG activities. The recombinant protein has the same substrate specificity as the chicken embryo 5-MCDG where hemimethylated DNA is a better substrate than symmetrically methylated CpGs. The activity ratio of G\/T mismatch and 5-MCDG is about 30:1 for the recombinant protein expressed in\n                    <jats:italic>E. coli<\/jats:italic>\n                    and 3:1 for the purified enzyme from chicken embryos. The incubation of a recombinant CpG-rich RNA isolated from the purified DNA demethylation complex with the recombinant enzyme strongly inhibits G\/T mismatch glycosylase while slightly stimulating the activity of 5-MCDG. Deletion mutations indicate that G\/T mismatch and 5-MCDG activities share the same areas of the N- and C-terminal parts of the protein. In reconstitution experiments RNA helicase in the presence of recombinant RNA and ATP potentiates the activity of 5-MCDG.\n                  <\/jats:p>","DOI":"10.1073\/pnas.100107597","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:52Z","timestamp":1027680052000},"page":"5135-5139","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":126,"title":["5-Methylcytosine-DNA glycosylase activity is present in a cloned G\/T mismatch DNA glycosylase associated with the chicken embryo DNA demethylation complex"],"prefix":"10.1073","volume":"97","author":[{"given":"Bing","family":"Zhu","sequence":"first","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Hess","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Angliker","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Siegmann","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Thiry","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Jost","sequence":"additional","affiliation":[{"name":"Friedrich Miescher-Institut, Maulbeerstrasse 66, CH-4058 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2000,4,25]]},"reference":[{"key":"e_1_3_4_1_2","volume-title":"Epigenetic Mechanisms of Gene Regulation","author":"Russo V E A","year":"1996","unstructured":"V E A Russo, R A Martienssen, A D Riggs Epigenetic Mechanisms of Gene Regulation (Cold Spring Harbor Lab. 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