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Cultures of the Cel48S deletion mutant (S mutant) were able to completely solubilize 10 g\/L crystalline cellulose. The cellulose hydrolysis rate of the S mutant strain was 60% lower than the parent strain, with the S mutant strain also exhibiting a 40% reduction in cell yield. The cellulosome produced by the S mutant strain was purified by affinity digestion, characterized enzymatically, and found to have a 35% lower specific activity on Avicel. The composition of the purified cellulosome was analyzed by tandem mass spectrometry with APEX quantification and no significant changes in abundance were observed in any of the major (&gt;1% of cellulosomal protein) enzymatic subunits. Although most cellulolytic bacteria have one family 48 cellulase,\n            <jats:italic>C. thermocellum<\/jats:italic>\n            has two, Cel48S and Cel48Y. Cellulose solubilization by a Cel48S and Cel48Y double knockout was essentially the same as that of the Cel48S single knockout. Our results indicate that solubilization of crystalline cellulose by\n            <jats:italic>C. thermocellum<\/jats:italic>\n            can proceed to completion without expression of a family 48 cellulase.\n          <\/jats:p>","DOI":"10.1073\/pnas.1003584107","type":"journal-article","created":{"date-parts":[[2010,9,14]],"date-time":"2010-09-14T03:02:19Z","timestamp":1284433339000},"page":"17727-17732","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["Deletion of the Cel48S cellulase from\n            <i>Clostridium thermocellum<\/i>"],"prefix":"10.1073","volume":"107","author":[{"given":"Daniel G.","family":"Olson","sequence":"first","affiliation":[{"name":"Mascoma Corporation, Lebanon, NH 03766;"},{"name":"Thayer School of Engineering and"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Shital A.","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Mascoma Corporation, Lebanon, NH 03766;"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Richard J.","family":"Giannone","sequence":"additional","affiliation":[{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"},{"name":"Oak Ridge National Laboratory, Oak Ridge, TN 37830"}]},{"given":"Jonathan","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Dartmouth College, Hanover, NH 03755;"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Nicky C.","family":"Caiazza","sequence":"additional","affiliation":[{"name":"Mascoma Corporation, Lebanon, NH 03766;"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"David A.","family":"Hogsett","sequence":"additional","affiliation":[{"name":"Mascoma Corporation, Lebanon, NH 03766;"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Robert L.","family":"Hettich","sequence":"additional","affiliation":[{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"},{"name":"Oak Ridge National Laboratory, Oak Ridge, TN 37830"}]},{"given":"Adam M.","family":"Guss","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering and"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Genia","family":"Dubrovsky","sequence":"additional","affiliation":[{"name":"Thayer School of Engineering and"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]},{"given":"Lee R.","family":"Lynd","sequence":"additional","affiliation":[{"name":"Mascoma Corporation, Lebanon, NH 03766;"},{"name":"Thayer School of Engineering and"},{"name":"Department of Biological Sciences, Dartmouth College, Hanover, NH 03755;"},{"name":"BioEnergy Science Center, Oak Ridge, TN 37830; and"}]}],"member":"341","published-online":{"date-parts":[[2010,9,13]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/nbt0208-169","article-title":"How biotech can transform biofuels","volume":"26","author":"Lynd LR","year":"2008","unstructured":"LR Lynd, et al., How biotech can transform biofuels. 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Microbiol Mol Biol Rev 66, 506\u2013577 (2002).","journal-title":"Microbiol Mol Biol Rev"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1128\/aem.43.5.1125-1132.1982","article-title":"Saccharification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum","volume":"43","author":"Johnson EA","year":"1982","unstructured":"EA Johnson, M Sakajoh, G Halliwell, A Madia, AL Demain, Saccharification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum. Appl Environ Microbiol 43, 1125\u20131132 (1982).","journal-title":"Appl Environ Microbiol"},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/MMBR.69.1.124-154.2005","article-title":"Cellulase, clostridia, and ethanol","volume":"69","author":"Demain AL","year":"2005","unstructured":"AL Demain, M Newcomb, JHD Wu, Cellulase, clostridia, and ethanol. 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J Bacteriol 156, 828\u2013836 (1983).","journal-title":"J Bacteriol"},{"key":"e_1_3_4_7_2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1574-6968.2006.00583.x","article-title":"Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically","volume":"268","author":"Berger E","year":"2007","unstructured":"E Berger, D Zhang, VV Zverlov, WH Schwarz, Two noncellulosomal cellulases of Clostridium thermocellum, Cel9I and Cel48Y, hydrolyse crystalline cellulose synergistically. 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