{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T11:10:41Z","timestamp":1769512241428,"version":"3.49.0"},"reference-count":22,"publisher":"Proceedings of the National Academy of Sciences","issue":"50","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,12,14]]},"abstract":"<jats:p>\n            To cause rice blast disease, the fungus\n            <jats:italic>Magnaporthe oryzae<\/jats:italic>\n            breaches the tough outer cuticle of the rice leaf by using specialized infection structures called appressoria. These cells allow the fungus to invade the host plant and proliferate rapidly within leaf tissue. Here, we show that a unique NADPH-dependent genetic switch regulates plant infection in response to the changing nutritional and redox conditions encountered by the pathogen. The biosynthetic enzyme trehalose-6-phosphate synthase (Tps1) integrates control of glucose-6-phosphate metabolism and nitrogen source utilization by regulating the oxidative pentose phosphate pathway, the generation of NADPH, and the activity of nitrate reductase. We report that Tps1 directly binds to NADPH and, thereby, regulates a set of related transcriptional corepressors, comprising three proteins, Nmr1, Nmr2, and Nmr3, which can each bind NADP. Targeted deletion of any of the Nmr-encoding genes partially suppresses the nonpathogenic phenotype of a \u0394\n            <jats:italic>tps1<\/jats:italic>\n            mutant. Tps1-dependent Nmr corepressors control the expression of a set of virulence-associated genes that are derepressed during appressorium-mediated plant infection. When considered together, these results suggest that initiation of rice blast disease by\n            <jats:italic>M. oryzae<\/jats:italic>\n            requires a regulatory mechanism involving an NADPH sensor protein, Tps1, a set of NADP-dependent transcriptional corepressors, and the nonconsuming interconversion of NADPH and NADP acting as signal transducer.\n          <\/jats:p>","DOI":"10.1073\/pnas.1006839107","type":"journal-article","created":{"date-parts":[[2010,11,30]],"date-time":"2010-11-30T09:18:15Z","timestamp":1291108695000},"page":"21902-21907","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":135,"title":["An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus"],"prefix":"10.1073","volume":"107","author":[{"given":"Richard A.","family":"Wilson","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom; and"},{"name":"Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722"}]},{"given":"Robert P.","family":"Gibson","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom; and"}]},{"given":"Cristian F.","family":"Quispe","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722"}]},{"given":"Jennifer A.","family":"Littlechild","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom; and"}]},{"given":"Nicholas J.","family":"Talbot","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Exeter, Exeter EX4 4QD, United Kingdom; and"}]}],"member":"341","published-online":{"date-parts":[[2010,11,29]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2032"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.327.5967.804"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg018"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601795"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/BF02174113","article-title":"NUT1, a major nitrogen regulatory gene in Magnaporthe grisea, is dispensable for pathogenicity","volume":"251","author":"Froeliger EH","year":"1996","unstructured":"EH Froeliger, BE Carpenter, NUT1, a major nitrogen regulatory gene in Magnaporthe grisea, is dispensable for pathogenicity. Mol Gen Genet 251, 647\u2013656 (1996).","journal-title":"Mol Gen Genet"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2307\/3870280"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.586-596.1998"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03449"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304104200"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.04958904"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.06.027"},{"key":"e_1_3_3_12_2","first-page":"1575","article-title":"Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea","volume":"5","author":"Talbot NJ","year":"1993","unstructured":"NJ Talbot, DJ Ebbole, JE Hamer, Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5, 1575\u20131590 (1993).","journal-title":"Plant Cell"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-3-135"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi000886j"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(97)01110-9"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00570-7"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151903"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08197"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.046300"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.2002.01220.x"},{"key":"e_1_3_3_21_2","volume-title":"Molecular Cloning: A laboratory manual","author":"Sambrook J","year":"1989","unstructured":"J Sambrook, EF Fritsch, T Maniatis Molecular Cloning: A laboratory manual (Cold Spring Harbor Lab Press, Cold Spring Harbor, NY, 1989)."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901477106"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1006839107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T22:55:57Z","timestamp":1649804157000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1006839107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,29]]},"references-count":22,"journal-issue":{"issue":"50","published-print":{"date-parts":[[2010,12,14]]}},"alternative-id":["10.1073\/pnas.1006839107"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1006839107","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,29]]},"assertion":[{"value":"2010-11-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}