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The outer glycoprotein shell of a mature WNV particle is formed by 30 rafts of three homodimers of the viral surface protein E. CR4354 binds to a discontinuous epitope formed by protein segments from two neighboring E molecules, but does not cause any detectable structural disturbance on the viral surface. The epitope occurs at two independent positions within an icosahedral asymmetric unit, resulting in 120 binding sites on the viral surface. The cross-linking of the six E monomers within one raft by four CR4354 Fab fragments suggests that the antibody neutralizes WNV by blocking the pH-induced rearrangement of the E protein required for virus fusion with the endosomal membrane.<\/jats:p>","DOI":"10.1073\/pnas.1011036107","type":"journal-article","created":{"date-parts":[[2010,10,19]],"date-time":"2010-10-19T04:19:23Z","timestamp":1287461963000},"page":"18950-18955","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":123,"title":["Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354"],"prefix":"10.1073","volume":"107","author":[{"given":"B\u00e4rbel","family":"Kaufmann","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew R.","family":"Vogt","sequence":"additional","affiliation":[{"name":"Departments of bPathology and Immunology,"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaap","family":"Goudsmit","sequence":"additional","affiliation":[{"name":"Crucell Holland BV, 2301 CA Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather A.","family":"Holdaway","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia A.","family":"Aksyuk","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Chipman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J.","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Departments of bPathology and Immunology,"},{"name":"Molecular Microbiology, and"},{"name":"Medicine, The Washington University School of Medicine, St. Louis, MO 63110; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Rossmann","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-2054;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2010,10,18]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1016\/j.mcna.2008.07.004","article-title":"West Nile virus in the Americas","volume":"92","author":"Petersen LR","year":"2008","unstructured":"LR Petersen, EB Hayes, West Nile virus in the Americas. 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