{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:07:14Z","timestamp":1780056434100,"version":"3.54.0"},"reference-count":37,"publisher":"Proceedings of the National Academy of Sciences","issue":"11","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,5,28]]},"abstract":"<jats:p>\n            Hormones, neurotransmitters, and growth factors give rise to calcium entry via receptor-activated cation channels that are activated downstream of phospholipase C activity. Members of the transient receptor potential channel (TRPC) family have been characterized as molecular substrates mediating receptor-activated cation influx. TRPC channels are assumed to be composed of multiple TRPC proteins. However, the cellular principles governing the assembly of TRPC proteins into homo- or heteromeric ion channels still remain elusive. By pursuing four independent experimental approaches\u2014i.e., subcellular cotrafficking of TRPC subunits, differential functional suppression by dominant-negative subunits, fluorescence resonance energy transfer between labeled TRPC subunits, and coimmunoprecipitation\u2014we investigate the combinatorial rules of TRPC assembly. Our data show that (\n            <jats:italic>i<\/jats:italic>\n            ) TRPC2 does not interact with any known TRPC protein and (\n            <jats:italic>ii<\/jats:italic>\n            ) TRPC1 has the ability to form channel complexes together with TRPC4 and TRPC5. (\n            <jats:italic>iii<\/jats:italic>\n            ) All other TRPCs exclusively assemble into homo- or heterotetramers within the confines of TRPC subfamilies\u2014e.g., TRPC4\/5 or TRPC3\/6\/7. The principles of TRPC channel formation offer the conceptual framework to assess the physiological role of distinct TRPC proteins in living cells.\n          <\/jats:p>","DOI":"10.1073\/pnas.102596199","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:46:49Z","timestamp":1027694809000},"page":"7461-7466","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":649,"title":["Subunit composition of mammalian transient receptor potential channels in living cells"],"prefix":"10.1073","volume":"99","author":[{"given":"Thomas","family":"Hofmann","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Philipps-Universit\u00e4t Marburg, Karl-von-Frisch-Strasse 1, D-35033 Marburg, Germany; and Institut f\u00fcr Pharmakologie, Freie Universit\u00e4t Berlin, Thielallee 67-73, D-14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Schaefer","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Philipps-Universit\u00e4t Marburg, Karl-von-Frisch-Strasse 1, D-35033 Marburg, Germany; and Institut f\u00fcr Pharmakologie, Freie Universit\u00e4t Berlin, Thielallee 67-73, D-14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"Schultz","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Philipps-Universit\u00e4t Marburg, Karl-von-Frisch-Strasse 1, D-35033 Marburg, Germany; and Institut f\u00fcr Pharmakologie, Freie Universit\u00e4t Berlin, Thielallee 67-73, D-14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gudermann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Philipps-Universit\u00e4t Marburg, Karl-von-Frisch-Strasse 1, D-35033 Marburg, Germany; and Institut f\u00fcr Pharmakologie, Freie Universit\u00e4t Berlin, Thielallee 67-73, D-14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,5,14]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80510-3"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90069-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.21.9652"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81233-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01004.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4274"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.10.5791"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.39.27359"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001099900070"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(99)01532-5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/35077544"},{"key":"e_1_3_3_12_2","volume-title":"Science's STKE","author":"Montell C","year":"2001","unstructured":"C Montell Science's STKE, ;2001\/90\/re1. 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