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More importantly, we show that  caveolin-2 is phosphorylated\n            <jats:italic>in vivo<\/jats:italic>\n            at two serine residues and that  the phosphorylation of caveolin-2 is necessary for its actions as a positive  regulator of caveolin-1 during organelle biogenesis in prostate cancer cells.  Mutation of the primary phosphorylation sites on caveolin-2, serine 23 and 36,  reduces the number of plasmalemma-attached caveolae and increases the  accumulation of noncoated vesicles, but does not affect trafficking of  caveolin-2, interaction with caveolin-1 or its biophysical properties. Thus,  the phosphorylation of caveolin-2 is a novel mechanism to regulate the  dynamics of caveolae assembly.\n          <\/jats:p>","DOI":"10.1073\/pnas.1031672100","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T17:10:32Z","timestamp":1054055432000},"page":"6511-6516","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":84,"title":["The phosphorylation of caveolin-2 on serines 23 and 36 modulates  caveolin-1-dependent caveolae formation"],"prefix":"10.1073","volume":"100","author":[{"given":"Grzegorz","family":"Sowa","sequence":"first","affiliation":[{"name":"Departments of Pharmacology, and Cell Biology, Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"}]},{"given":"Marc","family":"Pypaert","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology, and Cell Biology, Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"}]},{"given":"David","family":"Fulton","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology, and Cell Biology, Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"}]},{"given":"William C.","family":"Sessa","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology, and Cell Biology, Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536"}]}],"member":"341","published-online":{"date-parts":[[2003,5,12]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"1424","volume":"24","year":"1953","unstructured":"Palade, G. 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