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U.S.A."],"published-print":{"date-parts":[[2003,5,27]]},"abstract":"<jats:p>\n            In plants, the formation of isopentenyl diphosphate and dimethylallyl  diphosphate, the central intermediates in the biosynthesis of isoprenoids, is  compartmentalized: the mevalonate (MVA) pathway, which is localized to the  cytosol, is responsible for the synthesis of sterols, certain sesquiterpenes,  and the side chain of ubiquinone; in contrast, the recently discovered  MVA-independent pathway, which operates in plastids, is involved in providing  the precursors for monoterpenes, certain sesquiterpenes, diterpenes,  carotenoids, and the side chains of chlorophylls and plastoquinone. Specific  inhibitors of the MVA pathway (lovastatin) and the MVA-independent pathway  (fosmidomycin) were used to perturb biosynthetic flux in\n            <jats:italic>Arabidopsis  thaliana<\/jats:italic>\n            seedlings. The interaction between both pathways was studied at  the transcriptional level by using GeneChip (Affymetrix) microarrays and at  the metabolite level by assaying chlorophylls, carotenoids, and sterols.  Treatment of seedlings with lovastatin resulted in a transient decrease in  sterol levels and a transient increase in carotenoid as well as chlorophyll  levels. After the initial drop, sterol amounts in lovastatin-treated seedlings  recovered to levels above controls. As a response to fosmidomycin treatment, a  transient increase in sterol levels was observed, whereas chlorophyll and  carotenoid amounts decreased dramatically when compared with controls. At 96 h  after fosmidomycin addition, the levels of all metabolites assayed (sterols,  chlorophylls, and carotenoids) were substantially lower than in controls.  Interestingly, these inhibitor-mediated changes were not reflected in altered  gene expression levels of the genes involved in sterol, chlorophyll, and  carotenoid metabolism. The lack of correlation between gene expression  patterns and the accumulation of isoprenoid metabolites indicates that  posttranscriptional processes may play an important role in regulating flux  through isoprenoid metabolic pathways.\n          <\/jats:p>","DOI":"10.1073\/pnas.1031755100","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T17:10:32Z","timestamp":1054055432000},"page":"6866-6871","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":573,"title":["Crosstalk between cytosolic and plastidial pathways of isoprenoid  biosynthesis in Arabidopsis thaliana"],"prefix":"10.1073","volume":"100","author":[{"given":"Oliver","family":"Laule","sequence":"first","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"},{"name":"Institute of Plant Sciences, Swiss Federal Institute of Technology, LFW E57.1,  Universit\u00e4tsstrasse 2, CH-8092 Z\u00fcrich, Switzerland"}]},{"given":"Andreas","family":"F\u00fcrholz","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, Swiss Federal Institute of Technology, LFW E57.1,  Universit\u00e4tsstrasse 2, CH-8092 Z\u00fcrich, Switzerland"}]},{"given":"Hur-Song","family":"Chang","sequence":"additional","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"}]},{"given":"Tong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"}]},{"given":"Xun","family":"Wang","sequence":"additional","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"}]},{"given":"Peter B.","family":"Heifetz","sequence":"additional","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"}]},{"given":"Wilhelm","family":"Gruissem","sequence":"additional","affiliation":[{"name":"Institute of Plant Sciences, Swiss Federal Institute of Technology, LFW E57.1,  Universit\u00e4tsstrasse 2, CH-8092 Z\u00fcrich, Switzerland"}]},{"given":"Markus","family":"Lange","sequence":"additional","affiliation":[{"name":"Torrey Mesa Research Institute, 3115 Merryfield Row, San Diego, CA 92121"}]}],"member":"341","published-online":{"date-parts":[[2003,5,14]]},"reference":[{"key":"e_1_3_2_1_2","unstructured":"Harborne J. 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