{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T23:16:11Z","timestamp":1773443771075,"version":"3.50.1"},"reference-count":41,"publisher":"Proceedings of the National Academy of Sciences","issue":"32","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2012,8,7]]},"abstract":"<jats:p>\n            Embryogenesis requires the timely and coordinated activation of developmental regulators. It has been suggested that the recently discovered class of histone demethylases (UTX and JMJD3) that specifically target the repressive H3K27me3 modification play an important role in the activation of \u201cbivalent\u201d genes in response to specific developmental cues. To determine the requirements for UTX in pluripotency and development, we have generated\n            <jats:italic>Utx<\/jats:italic>\n            -null ES cells and mutant mice. The loss of UTX had a profound effect during embryogenesis.\n            <jats:italic>Utx<\/jats:italic>\n            -null embryos had reduced somite counts, neural tube closure defects and heart malformation that presented between E9.5 and E13.5. Unexpectedly, homozygous mutant female embryos were more severely affected than hemizygous mutant male embryos. In fact, we observed the survival of a subset of UTX-deficient males that were smaller in size and had reduced lifespan. Interestingly, these animals were fertile with normal spermatogenesis. Consistent with a midgestation lethality, UTX-null male and female ES cells gave rise to all three germ layers in teratoma assays, though sex-specific differences could be observed in the activation of developmental regulators in embryoid body assays. Lastly, ChIP-seq analysis revealed an increase in H3K27me3 in\n            <jats:italic>Utx<\/jats:italic>\n            -null male ES cells. In summary, our data demonstrate sex-specific requirements for this X-linked gene while suggesting a role for UTY during development.\n          <\/jats:p>","DOI":"10.1073\/pnas.1210787109","type":"journal-article","created":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T04:48:19Z","timestamp":1343105299000},"page":"13004-13009","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":178,"title":["X-linked H3K27me3 demethylase Utx is required for embryonic development in a sex-specific manner"],"prefix":"10.1073","volume":"109","author":[{"given":"G. Grant","family":"Welstead","sequence":"first","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"}]},{"given":"Menno P.","family":"Creyghton","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"}]},{"given":"Steve","family":"Bilodeau","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"}]},{"given":"Albert W.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"},{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142; and"},{"name":"Computational and Systems Biology Program, Massachusetts Institute of Technology, Cambridge, MA 02142"}]},{"given":"Styliani","family":"Markoulaki","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"}]},{"given":"Richard A.","family":"Young","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"},{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142; and"}]},{"given":"Rudolf","family":"Jaenisch","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142;"},{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142; and"}]}],"member":"341","published-online":{"date-parts":[[2012,7,23]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cell.2007.05.042","article-title":"A chromatin landmark and transcription initiation at most promoters in human cells","volume":"130","author":"Guenther MG","year":"2007","unstructured":"MG Guenther, SS Levine, LA Boyer, R Jaenisch, RA Young, A chromatin landmark and transcription initiation at most promoters in human cells. 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Mol Cell 40, 594\u2013605 (2010).","journal-title":"Mol Cell"},{"key":"e_1_3_4_26_2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1002\/jcb.22703","article-title":"Jmjd3 activates Mash1 gene in RA-induced neuronal differentiation of P19 cells","volume":"110","author":"Dai JP","year":"2010","unstructured":"JP Dai, JY Lu, Y Zhang, YF Shen, Jmjd3 activates Mash1 gene in RA-induced neuronal differentiation of P19 cells. J Cell Biochem 110, 1457\u20131463 (2010).","journal-title":"J Cell Biochem"},{"key":"e_1_3_4_27_2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nature06270","article-title":"SMRT-mediated repression of an H3K27 demethylase in progression from neural stem cell to neuron","volume":"450","author":"Jepsen K","year":"2007","unstructured":"K Jepsen, et al., SMRT-mediated repression of an H3K27 demethylase in progression from neural stem cell to neuron. Nature 450, 415\u2013419 (2007).","journal-title":"Nature"},{"key":"e_1_3_4_28_2","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/nbt.1562","article-title":"Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases","volume":"27","author":"Hockemeyer D","year":"2009","unstructured":"D Hockemeyer, et al., Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol 27, 851\u2013857 (2009).","journal-title":"Nat Biotechnol"},{"key":"e_1_3_4_29_2","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nbt.1927","article-title":"Genetic engineering of human pluripotent cells using TALE nucleases","volume":"29","author":"Hockemeyer D","year":"2011","unstructured":"D Hockemeyer, et al., Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol 29, 731\u2013734 (2011).","journal-title":"Nat Biotechnol"},{"key":"e_1_3_4_30_2","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/nature08672","article-title":"Systematic sequencing of renal carcinoma reveals inactivation of histone modifying genes","volume":"463","author":"Dalgliesh GL","year":"2010","unstructured":"GL Dalgliesh, et al., Systematic sequencing of renal carcinoma reveals inactivation of histone modifying genes. Nature 463, 360\u2013363 (2010).","journal-title":"Nature"},{"key":"e_1_3_4_31_2","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/ng.907","article-title":"Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder","volume":"43","author":"Gui Y","year":"2011","unstructured":"Y Gui, et al., Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder. Nat Genet 43, 875\u2013878 (2011).","journal-title":"Nat Genet"},{"key":"e_1_3_4_32_2","doi-asserted-by":"crossref","first-page":"3932","DOI":"10.1182\/blood-2010-10-311019","article-title":"Mutational spectrum analysis of chronic myelomonocytic leukemia includes genes associated with epigenetic regulation: UTX, EZH2, and DNMT3A","volume":"118","author":"Jankowska AM","year":"2011","unstructured":"AM Jankowska, et al., Mutational spectrum analysis of chronic myelomonocytic leukemia includes genes associated with epigenetic regulation: UTX, EZH2, and DNMT3A. Blood 118, 3932\u20133941 (2011).","journal-title":"Blood"},{"key":"e_1_3_4_33_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/ng.518","article-title":"Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin","volume":"42","author":"Morin RD","year":"2010","unstructured":"RD Morin, et al., Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin. Nat Genet 42, 181\u2013185 (2010).","journal-title":"Nat Genet"},{"key":"e_1_3_4_34_2","doi-asserted-by":"crossref","first-page":"897","DOI":"10.2217\/fon.10.50","article-title":"Genetic heterogeneity and chromatin modifiers in renal clear cell carcinoma","volume":"6","author":"Staller P","year":"2010","unstructured":"P Staller, Genetic heterogeneity and chromatin modifiers in renal clear cell carcinoma. Future Oncol 6, 897\u2013900 (2010).","journal-title":"Future Oncol"},{"key":"e_1_3_4_35_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/ng.349","article-title":"Somatic mutations of the histone H3K27 demethylase gene UTX in human cancer","volume":"41","author":"van Haaften G","year":"2009","unstructured":"G van Haaften, et al., Somatic mutations of the histone H3K27 demethylase gene UTX in human cancer. Nat Genet 41, 521\u2013523 (2009).","journal-title":"Nat Genet"},{"key":"e_1_3_4_36_2","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1172\/JCI45284","article-title":"Sequencing a mouse acute promyelocytic leukemia genome reveals genetic events relevant for disease progression","volume":"121","author":"Wartman LD","year":"2011","unstructured":"LD Wartman, et al., Sequencing a mouse acute promyelocytic leukemia genome reveals genetic events relevant for disease progression. J Clin Invest 121, 1445\u20131455 (2011).","journal-title":"J Clin Invest"},{"key":"e_1_3_4_37_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nature09784","article-title":"The Polycomb complex PRC2 and its mark in life","volume":"469","author":"Margueron R","year":"2011","unstructured":"R Margueron, D Reinberg, The Polycomb complex PRC2 and its mark in life. Nature 469, 343\u2013349 (2011).","journal-title":"Nature"},{"key":"e_1_3_4_38_2","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1073\/pnas.101118898","article-title":"Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation","volume":"98","author":"Eggan K","year":"2001","unstructured":"K Eggan, et al., Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc Natl Acad Sci USA 98, 6209\u20136214 (2001).","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_3_4_39_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ymeth.2008.04.002","article-title":"Somatic cell nuclear transfer and derivation of embryonic stem cells in the mouse","volume":"45","author":"Markoulaki S","year":"2008","unstructured":"S Markoulaki, A Meissner, R Jaenisch, Somatic cell nuclear transfer and derivation of embryonic stem cells in the mouse. Methods 45, 101\u2013114 (2008).","journal-title":"Methods"},{"key":"e_1_3_4_40_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/5669","article-title":"Neurotrophin-3 is required for proper cerebellar development","volume":"2","author":"Bates B","year":"1999","unstructured":"B Bates, et al., Neurotrophin-3 is required for proper cerebellar development. Nat Neurosci 2, 115\u2013117 (1999).","journal-title":"Nat Neurosci"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/dvg.20226","article-title":"Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre\/loxP mediated recombination in the nervous system, kidney, and somite-derived tissues","volume":"44","author":"Dubois NC","year":"2006","unstructured":"NC Dubois, D Hofmann, K Kaloulis, JM Bishop, A Trumpp, Nestin-Cre transgenic mouse line Nes-Cre1 mediates highly efficient Cre\/loxP mediated recombination in the nervous system, kidney, and somite-derived tissues. Genesis 44, 355\u2013360 (2006).","journal-title":"Genesis"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1210787109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T08:51:25Z","timestamp":1654591885000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1210787109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,23]]},"references-count":41,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2012,8,7]]}},"alternative-id":["10.1073\/pnas.1210787109"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1210787109","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,23]]},"assertion":[{"value":"2012-07-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}