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Two widely distributed isoenzyme types of\n            <jats:italic>T. cruzi<\/jats:italic>\n            are hybrids, their genetic composition being the likely result of genetic exchange between two distantly related lineages. The data show that the reference strain for the\n            <jats:italic>T. cruzi<\/jats:italic>\n            genome project (CL Brener) is a hybrid. Well-supported gene genealogies show that mitochondrial and nuclear gene sequences from\n            <jats:italic>T. cruzi<\/jats:italic>\n            cluster, respectively, in three or four distinct clades that do not fully correspond to the two previously defined major lineages of\n            <jats:italic>T. cruzi<\/jats:italic>\n            . There is clear genetic differentiation among the major groups of sequences, but genetic diversity within each major group is low. We estimate that the major extant lineages of\n            <jats:italic>T. cruzi<\/jats:italic>\n            have diverged during the Miocene or early Pliocene (3\u201316 million years ago).\n          <\/jats:p>","DOI":"10.1073\/pnas.121187198","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:45:09Z","timestamp":1027694709000},"page":"7396-7401","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":266,"title":["Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of\n            <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1073","volume":"98","author":[{"given":"Carlos A.","family":"Machado","sequence":"first","affiliation":[{"name":"Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco 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