{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:19:32Z","timestamp":1781104772941,"version":"3.54.1"},"reference-count":28,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,6,20]]},"abstract":"<jats:p>\n                    MEK kinase 1 (MEKK1) is a 196-kDa mitogen-activated protein kinase (MAPK) kinase kinase that, in addition to regulating the c-Jun NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal kinase (JNK) pathway, is involved in the control of cell motility. MEKK1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice are defective in eyelid closure, a TGF\u03b1-directed process involving the migration of epithelial cells. MEKK1 expression in epithelial cells stimulates lamellipodia formation, a process required for cell movement. In addition, mouse embryo fibroblasts derived from MEKK1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice are inhibited in their migration relative to MEKK1\n                    <jats:sup>+\/+<\/jats:sup>\n                    fibroblasts. MEKK1 is required for JNK but not NF-\u03baB activation in response to virus infection, microtubule disruption, and stimulation of embryonic stem cells with lysophosphatidic acid. MEKK1 is not required for TNF\u03b1 or IL-1 regulation of JNK or NF-\u03baB activation in macrophages or fibroblasts. Thus, MEKK1 senses microtubule integrity, contributes to the regulation of fibroblast and epithelial cell migration, and is required for activation of JNK but not NF-\u03baB in response to selected stress stimuli.\n                  <\/jats:p>","DOI":"10.1073\/pnas.130176697","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:37:36Z","timestamp":1027679856000},"page":"7272-7277","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":208,"title":["MEK kinase 1 gene disruption alters cell migration and c-Jun NH\n                    <sub>2<\/sub>\n                    -terminal kinase regulation but does not cause a measurable defect in NF-\u03baB activation"],"prefix":"10.1073","volume":"97","author":[{"given":"Toshiaki","family":"Yujiri","sequence":"first","affiliation":[{"name":"Third Department of Internal Medicine, Yamaguchi University School of Medicine, Yamaguchi, Japan; Program in Molecular 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