{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T03:20:42Z","timestamp":1769311242633,"version":"3.49.0"},"reference-count":42,"publisher":"Proceedings of the National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,6,20]]},"abstract":"<jats:p>Centromere protein A (CENP-A) is a variant of histone H3 with more than 60% sequence identity at the C-terminal histone fold domain. CENP-A specifically locates to active centromeres of animal chromosomes and therefore is believed to be a component of the specialized centromeric nucleosomes on which the kinetochores are assembled. Here we report that CENP-A, highly purified from HeLa cells, can indeed replace histone H3 in a nucleosome reconstitution system mediated by nucleosome assembly protein-1 (NAP-1). The structure of the nucleosomes reconstituted with recombinant CENP-A, histones H2A, H2B, and H4, and closed circular DNAs had the following properties. By atomic force microscopy, \u201cbeads on a string\u201d images were obtained that were similar to those obtained with nucleosomes reconstituted with four standard histones. DNA ladders with repeats of approximately 10 bp were produced by DNase I digestion, indicating that the DNA was wrapped round the protein complex. Mononucleosomes isolated by glycerol gradient sedimentation had a relative molecular mass of \u2248200 kDa and were composed of 120\u2013150 bp of DNA and equimolar amounts of CENP-A, and histones H4, H2A, and H2B. Thus, we conclude that CENP-A forms an octameric complex with histones H4, H2A, and H2B in the presence of DNA.<\/jats:p>","DOI":"10.1073\/pnas.130189697","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:41:48Z","timestamp":1027694508000},"page":"7266-7271","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":194,"title":["Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution\n            <i>in vitro<\/i>"],"prefix":"10.1073","volume":"97","author":[{"given":"Kinya","family":"Yoda","sequence":"first","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Satoshi","family":"Ando","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Setsuo","family":"Morishita","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Kenichi","family":"Houmura","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Keiji","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Kunio","family":"Takeyasu","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science and..."}]},{"given":"Tuneko","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Bioscience Center, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan; Japan Bioindustry Association, Hatchobori 2-Chome, Chuo-ku, Tokyo 104-3302, Japan; Fujita Health University Institute for Comprehensive Medical Science, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan; Department of Natural Environment Science, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu, Kyoto 606-8501, Japan; and Core Research for Evolutional Science and Technology (CREST), Japan Science 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