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We find that the core biochemical processes are maintained, whereas the capacity to sense chemical ligands is diminished or obscured. We use the pathways for multiinput gene regulation and show that they can serve as a rich source of orthogonal building blocks for gene expression control in mammalian cells. Our findings open new avenues in synthetic circuit design.<\/jats:p>","DOI":"10.1073\/pnas.1406482111","type":"journal-article","created":{"date-parts":[[2014,10,21]],"date-time":"2014-10-21T06:45:44Z","timestamp":1413873944000},"page":"15705-15710","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":56,"title":["Transplantation of prokaryotic two-component signaling pathways into mammalian cells"],"prefix":"10.1073","volume":"111","author":[{"given":"Jonathan","family":"Hansen","sequence":"first","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]},{"given":"Erik","family":"Mailand","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]},{"given":"Krishna Kumar","family":"Swaminathan","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]},{"given":"Joerg","family":"Schreiber","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]},{"given":"Bartolomeo","family":"Angelici","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]},{"given":"Yaakov","family":"Benenson","sequence":"additional","affiliation":[{"name":"Department of Biosystems Science and Engineering, Eidgen\u00f6ssische Technische Hochschule Z\u00fcrich (ETH Zurich), CH-4058 Basel, Switzerland"}]}],"member":"341","published-online":{"date-parts":[[2014,10,20]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.cell.2010.12.014","article-title":"Tunable signal processing in synthetic MAP kinase cascades","volume":"144","author":"O\u2019Shaughnessy EC","year":"2011","unstructured":"EC O\u2019Shaughnessy, S Palani, JJ Collins, CA Sarkar, Tunable signal processing in synthetic MAP kinase cascades. 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