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These interactions prevent Gag proteins from effectively multimerizing into viral complexes at the plasma membrane and lead to inhibition of viral particle production. The microRNA\u2013Gag interactions further result in Gag proteins being redirected into the endocytic pathway where they are degraded in lysosomes. These findings have significant implications for understanding how cells modulate HIV-1 infection and raise the possibility of manipulating total expression levels of host miRNAs to combat HIV-1 replication.<\/jats:p>","DOI":"10.1073\/pnas.1408037111","type":"journal-article","created":{"date-parts":[[2014,6,18]],"date-time":"2014-06-18T04:24:18Z","timestamp":1403065458000},"update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":53,"title":["MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production"],"prefix":"10.1073","volume":"111","author":[{"given":"Antony K.","family":"Chen","sequence":"first","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"},{"name":"Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabuddha","family":"Sengupta","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayoko","family":"Waki","sequence":"additional","affiliation":[{"name":"Virus\u2013Cell Interaction Section, HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Schuyler B.","family":"Van Engelenburg","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Ochiya","sequence":"additional","affiliation":[{"name":"Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo 104-0045, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherimay D.","family":"Ablan","sequence":"additional","affiliation":[{"name":"Virus\u2013Cell Interaction Section, HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric O.","family":"Freed","sequence":"additional","affiliation":[{"name":"Virus\u2013Cell Interaction Section, HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Lippincott-Schwartz","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2014,6,17]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb785"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.384"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3979"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.10.6487-6497.1995"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091000398"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907364106"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001200"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209414109"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3113"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2210"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1639"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.06.003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr961"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06998"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504812102"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1176"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.12.5395-5402.2000"},{"key":"e_1_3_3_20_2","first-page":"373","article-title":"Diverse interactions of retroviral Gag proteins with RNAs","volume":"36","author":"Rein A","year":"2011","unstructured":"A Rein, SA Datta, CP Jones, K Musier-Forsyth, Diverse interactions of retroviral Gag proteins with RNAs. 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