{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:00:26Z","timestamp":1781103626306,"version":"3.54.1"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,6,19]]},"abstract":"<jats:p>\n                    Exit from mitosis in budding yeast requires inactivation of cyclin-dependent kinases through mechanisms triggered by the protein phosphatase Cdc14. Cdc14 activity, in turn, is regulated by a group of proteins, the mitotic exit network (MEN), which includes Lte1, Tem1, Cdc5, Cdc15, Dbf2\/Dbf20, and Mob1. The direct biochemical interactions between the components of the MEN remain largely unresolved. Here, we investigate the mechanisms that underlie activation of the protein kinase Dbf2. Dbf2 kinase activity depended on Tem1, Cdc15, and Mob1\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                    <jats:italic>In vitro<\/jats:italic>\n                    , recombinant protein kinase Cdc15 activated recombinant Dbf2, but only when Dbf2 was bound to Mob1. Conserved phosphorylation sites Ser-374 and Thr-544 (present in the human,\n                    <jats:italic>Caenorhabditis elegans,<\/jats:italic>\n                    and\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    relatives of Dbf2) were required for\n                    <jats:italic>DBF2<\/jats:italic>\n                    function\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and activation of Dbf2-Mob1 by Cdc15\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Although Cdc15 phosphorylated Dbf2, Dbf2\u2013Mob1, and Dbf2(S374A\/T544A)\u2013Mob1, the pattern of phosphate incorporation into Dbf2 was substantially altered by either the S374A T544A mutations or omission of Mob1. Thus, Cdc15 promotes the exit from mitosis by directly switching on the kinase activity of Dbf2. We propose that Mob1 promotes this activation process by enabling Cdc15 to phosphorylate the critical Ser-374 and Thr-544 phosphoacceptor sites of Dbf2.\n                  <\/jats:p>","DOI":"10.1073\/pnas.141098998","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"7325-7330","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":173,"title":["Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex"],"prefix":"10.1073","volume":"98","author":[{"given":"Angie S.","family":"Mah","sequence":"first","affiliation":[{"name":"Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Jang","sequence":"additional","affiliation":[{"name":"Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond J.","family":"Deshaies","sequence":"additional","affiliation":[{"name":"Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 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