{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:34:56Z","timestamp":1781105696339,"version":"3.54.1"},"reference-count":50,"publisher":"National Academy of Sciences","issue":"20","license":[{"start":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T00:00:00Z","timestamp":1430697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM098469"],"award-info":[{"award-number":["R01GM098469"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007288"],"award-info":[{"award-number":["T32GM007288"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM008572"],"award-info":[{"award-number":["T32GM008572"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2015,5,19]]},"abstract":"<jats:title>Significance<\/jats:title>\n                  <jats:p>Cytoplasmic dynein is the primary minus-end\u2013directed microtubule (MT) motor. It is unclear how dynein coordinates ATP hydrolysis and MT attachment within and between its two motor domains, each containing four AAA+ ATPases (AAA: ATPase associated with various cellular activities), AAA1\u20134. We characterize how mechanical tension and nucleotide states of AAA1 and AAA3 regulate dynein\u2013MT binding. Dynein binds MTs tighter when subjected to tension opposite its normal motility. ADP binding to AAA3 unexpectedly weakens MT-binding strength and reduces the bond strength anisotropy. Finally, AAA3 \u201cgates\u201d the activity of AAA1: ATP binding to AAA1 induces MT release only if AAA3 contains nucleotide. This work expands understanding of the role of force in dynein mechanochemistry and identifies regulatory functions of AAA3.<\/jats:p>","DOI":"10.1073\/pnas.1417422112","type":"journal-article","created":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T22:02:32Z","timestamp":1430776952000},"page":"6371-6376","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":119,"title":["Cytoplasmic dynein regulates its attachment to microtubules via nucleotide state-switched mechanosensing at multiple AAA domains"],"prefix":"10.1073","volume":"112","author":[{"given":"Matthew P.","family":"Nicholas","sequence":"first","affiliation":[{"name":"Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, and"},{"name":"Medical Scientist Training Program, Albert Einstein College of Medicine, Bronx, NY 10461;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Berger","sequence":"additional","affiliation":[{"name":"Theory and Bio-Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany;"},{"name":"Laboratory of Sensory Neuroscience, and"},{"name":"Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibylle","family":"Brenner","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Gennerich","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2015,5,4]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2420"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2804"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2272"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10955"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1555"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501636200"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb930"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.03372"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.018"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.05.046"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.016"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215804"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2205"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092546199"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.07.018"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb911"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510191103"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800564105"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002430107"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5587"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61574-1"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-10-0675"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi048985a"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403429101"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802951200"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2637011100"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78802-7"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1208349109"},{"key":"e_1_3_3_29_2","unstructured":"M Dembo On peeling an adherent cell from a surface. Lectures on Mathematics in the Life Sciences Some Mathematical Problems in Biology (American Mathematical Society Providence RI) Vol 24 pp 51\u201377. (1994)."},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.30.1.105"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1107841108"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2012.06.038"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.044"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2930"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M707815200"},{"key":"e_1_3_3_36_2","volume-title":"Practical Nonparametric Statistics","author":"Conover WJ","year":"1999","unstructured":"WJ Conover Practical Nonparametric Statistics (Wiley, 3rd Ed, New York, 1999).","edition":"3"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.10.061807.160427"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.37.032807.125804"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806085105"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702370104"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164424"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1224151"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.10.039"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.07.022"},{"key":"e_1_3_3_45_2","first-page":"705","article-title":"Crystal clear insights into how the dynein motor moves","volume":"126","author":"Carter AP","year":"2013","unstructured":"AP Carter, Crystal clear insights into how the dynein motor moves. J Cell Sci 126, 705\u2013713 (2013).","journal-title":"J Cell Sci"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2009.12.014"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0329-0_9"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907133106"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn301895c"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0329-0_10"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.pnas.org\/syndication\/doi\/10.1073\/pnas.1417422112","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.1417422112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:27:39Z","timestamp":1649809659000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.1417422112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,4]]},"references-count":50,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2015,5,19]]}},"alternative-id":["10.1073\/pnas.1417422112"],"URL":"https:\/\/doi.org\/10.1073\/pnas.1417422112","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,4]]},"assertion":[{"value":"2015-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}