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Within the dimer, the catalytic core domains form the only dimer interface, and the C-terminal domains are located 55 \u212b apart. A 26-aa \u03b1-helix, \u03b16, links the C-terminal domain to the catalytic core. A kink in one of the two \u03b16 helices occurs near a known proteolytic site, suggesting that it may act as a flexible elbow to reorient the domains during the integration process. Two proteins that bind DNA in a sequence-independent manner are structurally homologous to the HIV-1 IN C-terminal domain, suggesting a similar protein\u2013DNA interaction in which the IN C-terminal domain may serve to bind, bend, and orient viral DNA during integration. A strip of positively charged amino acids contributed by both monomers emerges from each active site of the dimer, suggesting a minimally dimeric platform for binding each viral DNA end. The crystal structure of the isolated catalytic core domain (residues 52\u2013210), independently determined at 1.6-\u212b resolution, is identical to the core domain within the two-domain 52\u2013288 structure.<\/jats:p>","DOI":"10.1073\/pnas.150220297","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:32:33Z","timestamp":1027679553000},"page":"8233-8238","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":352,"title":["Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: A model for viral DNA binding"],"prefix":"10.1073","volume":"97","author":[{"given":"Julian C.-H.","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of Biochemistry and Biophysics, Laboratory Medicine, and Internal Medicine, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Krucinski","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Biophysics, Laboratory Medicine, and Internal Medicine, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry J. 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