{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T06:48:39Z","timestamp":1776322119766,"version":"3.50.1"},"reference-count":31,"publisher":"Proceedings of the National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,10,23]]},"abstract":"<jats:p>\n            The surface glycoprotein of the Lassa virus, a member of the\n            <jats:italic>arenaviridae<\/jats:italic>\n            family, is synthesized as a 76-kDa precursor (GP-C) that is posttranslationally cleaved into an N-terminal 44-kDa subunit and a C-terminal membrane-anchored 36-kDa subunit. Cleavage occurs at the C-terminal end of the unusual recognition motif R-R-L-L. We show here that GP-C is cleaved in the endoplasmic reticulum by the cellular subtilase SKI-1\/S1P, an enzyme that has so far been observed to be involved in cholesterol metabolism. Furthermore, we present evidence that only cleaved glycoprotein is incorporated into virions and that this is necessary for the formation of infectious virus. To our knowledge, there have been no previous reports of this type of viral glycoprotein processing, one that may be an interesting target for antiviral therapy.\n          <\/jats:p>","DOI":"10.1073\/pnas.221447598","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:34:10Z","timestamp":1027694050000},"page":"12701-12705","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":318,"title":["The Lassa virus glycoprotein precursor GP-C is proteolytically processed by subtilase SKI-1\/S1P"],"prefix":"10.1073","volume":"98","author":[{"given":"Oliver","family":"Lenz","sequence":"first","affiliation":[{"name":"Institute of Virology, Philipps University, Robert-Koch-Strasse 17, 35037 Marburg, Germany; and Laboratory of Biochemical Neuroendocrinology, Cinical Research Institute of Montreal, Montreal, QC, Canada H2W 1R7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"ter Meulen","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Robert-Koch-Strasse 17, 35037 Marburg, Germany; and Laboratory of Biochemical Neuroendocrinology, Cinical Research Institute of Montreal, Montreal, QC, Canada H2W 1R7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Dieter","family":"Klenk","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Robert-Koch-Strasse 17, 35037 Marburg, Germany; and Laboratory of Biochemical Neuroendocrinology, Cinical Research Institute of Montreal, Montreal, QC, Canada H2W 1R7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabil G.","family":"Seidah","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Robert-Koch-Strasse 17, 35037 Marburg, Germany; and Laboratory of Biochemical Neuroendocrinology, Cinical Research Institute of Montreal, Montreal, QC, Canada H2W 1R7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Garten","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University, Robert-Koch-Strasse 17, 35037 Marburg, Germany; and Laboratory of Biochemical Neuroendocrinology, Cinical Research Institute of Montreal, Montreal, QC, Canada H2W 1R7"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2001,10,16]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.437"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.445"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00676.x"},{"key":"e_1_3_3_4_2","first-page":"1505","volume-title":"Fields Virology","author":"Southern P J","year":"1996","unstructured":"P J Southern Fields Virology, eds B N Fields, P M Knipe, Howley (Lippincott, Philadelphia), pp. 1505\u20131519 (1996)."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3028-2_2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5396.2079"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(92)90360-X"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.23.11418-11421.2000"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90123-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.32.22795"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80150-1"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.4.1321"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.43.28261"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.66.3.1495-1505.1992"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(84)90040-X"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)41980-6"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02231.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81668-2"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2349"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90685-5"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9284"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9084(94)90149-X"},{"key":"e_1_3_3_24_2","volume-title":"Cellular Receptors for Animal Viruses","author":"Klenk H D","year":"1994","unstructured":"H D Klenk, W Garten Cellular Receptors for Animal Viruses, ed E Wimmer (Cold Spring Harbor Lab. 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