{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T04:33:16Z","timestamp":1781238796922,"version":"3.54.1"},"reference-count":46,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,1,8]]},"abstract":"<jats:p>\n                    Histone methylation has emerged as an important mechanism for\n regulating the transcriptional accessibility of chromatin. Several\n methyltransferases have been shown to target histone amino-terminal\n tails and mark nucleosomes associated with either euchromatic or\n heterochromatic states. However, the biochemical machinery\n responsible for regulating histone methylation and integrating it with\n other cellular events has not been well characterized. We report here\n the purification, molecular identification, and genetic and biochemical\n characterization of the Set1 protein complex that is necessary for\n methylation of histone H3 at lysine residue 4 in\n                    <jats:italic>Saccharomyces\n cerevisiae<\/jats:italic>\n                    . The seven-member 363-kDa complex contains homologs\n of\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    proteins Ash2 and Trithorax\n and\n                    <jats:italic>Caenorhabditis<\/jats:italic>\n                    <jats:italic>elegans<\/jats:italic>\n                    protein DPY-30, which are implicated in\n the maintenance of\n                    <jats:italic>Hox<\/jats:italic>\n                    gene expression and regulation of\n X chromosome dosage compensation, respectively. Mutations of Set1\n protein comparable to those that disrupt developmental function of its\n                    <jats:italic>Drosophila<\/jats:italic>\n                    homolog Trithorax abrogate histone\n methylation in yeast. These studies suggest that epigenetic regulation\n of developmental and sex-specific gene expression are species-specific\n readouts for a common chromatin remodeling machinery associated\n mechanistically with histone methylation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.221596698","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:36:44Z","timestamp":1027679804000},"page":"90-94","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":275,"title":["A trithorax-group complex purified from\n                    <i>Saccharomyces cerevisiae<\/i>\n                    is required for methylation of histone H3"],"prefix":"10.1073","volume":"99","author":[{"given":"Peter L.","family":"Nagy","sequence":"first","affiliation":[{"name":"Departments of Pathology and Structural Biology,\r Stanford University School of Medicine, Stanford, CA 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