{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T14:48:14Z","timestamp":1772722094931,"version":"3.50.1"},"reference-count":24,"publisher":"Proceedings of the National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,11,6]]},"abstract":"<jats:p>\n            The functional role and specificity of tumor infiltrating lymphocytes (TIL) is generally not well characterized. Prominent lymphocyte infiltration is the hallmark of the most common form of hereditary colon cancer, hereditary nonpolyposis colon cancer (HNPCC) and the corresponding spontaneous colon cancers with the microsatellite instability (MSI) phenotype. These cancers are caused by inherited or acquired defects in the DNA mismatch\u2013repair machinery. The molecular mechanism behind the MSI phenotype provides a clue to understanding the lymphocyte reaction by allowing reliable prediction of potential T cell epitopes created by frameshift mutations in candidate genes carrying nucleotide repeat sequences, such as\n            <jats:italic>TGF\u03b2RII<\/jats:italic>\n            and\n            <jats:italic>BAX<\/jats:italic>\n            . These tumors therefore represent an interesting human system for studying TIL and characterizing tumor-specific T cells. We here describe T cell reactivity against several T helper cell epitopes, representing a common frameshift mutation in\n            <jats:italic>TGF\u03b2RII<\/jats:italic>\n            , in TIL and peripheral blood lymphocytes from patients with MSI\n            <jats:sup>+<\/jats:sup>\n            tumors. The peptide SLVRLSSCVPVALMSAMTTSSSQ was recognized by T cells from two of three patients with spontaneous MSI\n            <jats:sup>+<\/jats:sup>\n            colon cancers and from all three patients with HNPCC. Because such mutations are present in 90% of cancers within this patient group, these newly characterized epitopes provide attractive targets for cancer vaccines, including a prophylactic vaccine for individuals carrying a genetic disposition for developing HNPCC.\n          <\/jats:p>","DOI":"10.1073\/pnas.231326898","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:32:33Z","timestamp":1027693953000},"page":"13255-13260","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":225,"title":["Frameshift-mutation-derived peptides as tumor-specific antigens in inherited and spontaneous colorectal cancer"],"prefix":"10.1073","volume":"98","author":[{"given":"Ingvil","family":"S\u00e6terdal","sequence":"first","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Jens","family":"Bj\u00f8rheim","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Kari","family":"Lislerud","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Marianne K.","family":"Gjertsen","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Ida K.","family":"Bukholm","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Ole Christian","family":"Olsen","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Jahn M.","family":"Nesland","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Jon Amund","family":"Eriksen","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Mona","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Annika","family":"Lindblom","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]},{"given":"Gustav","family":"Gaudernack","sequence":"additional","affiliation":[{"name":"The Norwegian Radium Hospital, Department of Immunology, Section for Immunotherapy, and Department of Pathology, The Norwegian Radium Hospital, University of Oslo, 0310 Oslo, Norway; Department of Surgery, The Central Hospital of Akershus, 1474 Nordbyhagen, Norway; Department of Surgery, The Central Hospital of Buskerud, 3004 Drammen, Norway; Hydro Research Center, 3908 Porsgrunn, Norway; and Department of Molecular Medicine, Karolinska Institute, 17176 Stockholm, Sweden"}]}],"member":"341","published-online":{"date-parts":[[2001,10,30]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990315)80:6<827::AID-IJC6>3.0.CO;2-P"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/363558a0"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8484122"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81333-1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7761852"},{"key":"e_1_3_3_6_2","first-page":"5548","volume":"55","author":"Parsons R","year":"1995","unstructured":"R Parsons, L L Myeroff, B Liu, J K Willson, S D Markowitz, K W Kinzler, B Vogelstein Cancer Res 55, 5548\u20135550 (1995).","journal-title":"Cancer Res"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5302.967"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1298"},{"key":"e_1_3_3_9_2","first-page":"256","volume":"77","author":"Di Giorgio A","year":"1992","unstructured":"A Di Giorgio, C Botti, A Tocchi, P Mingazzini, M Flammia Int Surg 77, 256\u2013260 (1992).","journal-title":"Int Surg"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(96)00078-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(92)90334-J"},{"key":"e_1_3_3_12_2","first-page":"5330","volume":"55","author":"Bakker A B","year":"1995","unstructured":"A B Bakker, G Marland, A J de Boer, R J Huijbens, E H Danen, G J Adema, C G Figdor Cancer Res 55, 5330\u20135334 (1995).","journal-title":"Cancer Res"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980302)75:5<794::AID-IJC21>3.0.CO;2-5"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1996.638"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830231045"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.2144\/01305st01"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.9.2261"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.13.s8.29.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65436-3"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1993.290"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0395-231"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1299"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1840703"},{"key":"e_1_3_3_24_2","unstructured":"S\u00e6terdal I. Gjertsen M. K. thor Straten P. Eriksen J. A. & Gaudernack G. A. Cancer Immunol. Immunother . in press."}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.231326898","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:34:13Z","timestamp":1649853253000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.231326898"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,30]]},"references-count":24,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2001,11,6]]}},"alternative-id":["10.1073\/pnas.231326898"],"URL":"https:\/\/doi.org\/10.1073\/pnas.231326898","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,30]]},"assertion":[{"value":"2001-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}