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Because the sequencing templates were generated from primary AML cells, the technique favors the detection of mutations from the most dominant clones within the tumor cell mixture. This strategy represents a viable approach for the detection of potentially relevant, nonrandom mutations in primary human cancer cell genomes.<\/jats:p>","DOI":"10.1073\/pnas.2335924100","type":"journal-article","created":{"date-parts":[[2003,11,25]],"date-time":"2003-11-25T20:59:06Z","timestamp":1069793946000},"page":"14275-14280","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":50,"title":["A pilot study of high-throughput, sequence-based mutational profiling of primary human acute myeloid leukemia cell genomes"],"prefix":"10.1073","volume":"100","author":[{"given":"Timothy J.","family":"Ley","sequence":"first","affiliation":[{"name":"Departments of Medicine, Genetics, and Pathology and Immunology, Siteman Cancer Center, Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63110; Ohio State University Comprehensive Cancer Center and James Cancer Hospital, Columbus, OH 43210; University of Chicago, Chicago, IL 60637; 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