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Whereas na\u00efve T cells are resistant to adenoviral infection, Tg expression of CAR on T cells greatly facilitates adenoviral-mediated gene expression<jats:italic>ex vivo<\/jats:italic>,<jats:italic>in vivo<\/jats:italic>, and in differentiated T helper cells. Thus we have developed a technology for efficient gene delivery to na\u00efve T cells. By using adenoviral vectors encoding specific inhibitors, we show that G1 cyclin-dependent kinase, NF-\u03baB, and caspase activities are required for the proliferation of primary T cells. In addition, by expressing Bcl-x<jats:sub>L<\/jats:sub>protein at a level that closely approximates mitogen-induced levels, we demonstrate that Bcl-x<jats:sub>L<\/jats:sub>expression is sufficient to account for mitogen-mediated survival of primary T cells. Thus, adenoviral-mediated gene delivery to CAR Tg T cells should be useful for the analysis of many genes controlling T cell fate.<\/jats:p>","DOI":"10.1073\/pnas.250356297","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:44:19Z","timestamp":1027694659000},"page":"13784-13789","update-policy":"http:\/\/dx.doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":69,"title":["Transgenic expression of the coxsackie\/adenovirus receptor enables adenoviral-mediated gene delivery in na\u00efve T cells"],"prefix":"10.1073","volume":"97","author":[{"given":"Yisong Y.","family":"Wan","sequence":"first","affiliation":[{"name":"Departments of Biochemistry and Molecular Genetics and Pediatrics, and Microbiology, Program in Molecular Biology, University of Colorado Health Sciences Center, BRB802, 4200 East Ninth Avenue, Denver, CO 80262; and Departments of Pathology and Medicine, and Committees on Immunology and Cancer Biology, 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