{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:27:19Z","timestamp":1771003639194,"version":"3.50.1"},"reference-count":33,"publisher":"Proceedings of the National Academy of Sciences","issue":"26","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2000,12,19]]},"abstract":"<jats:p>\n            Gene delivery to the central nervous system is central to the\n development of gene therapy for neurological diseases. We developed a\n baculovirus-derived vector, the Bac-CMV-GFP vector, containing a\n reporter gene encoding for the green fluorescent protein (GFP) under\n the control of the cytomegalovirus (CMV) promoter. Two neuroblastomal\n cell lines and three human primary neural cultures could be efficiently\n transduced. In all cases, addition of butyrate, an inhibitor of histone\n deacetylase, increased the level of expression in terms of the number\n of GFP-expressing cells and the intensity of fluorescence. The level of\n expression in a human telencephalic culture was over 50% of transduced\n cells with a multiplicity of infection of 25. GFP expression was\n demonstrated to be genuine expression and not pseudotransduction of the\n reporter protein. Most interestingly, Bac-CMV-GFP could transduce\n neural cells\n            <jats:italic>in vivo<\/jats:italic>\n            when directly injected into the\n brain of rodents and was not inactivated by the complement system.\n Thus, baculovirus is a promising tool for gene transfer into the\n central nervous system both for studies of the function of foreign\n genes and the development of gene therapy strategies.\n          <\/jats:p>","DOI":"10.1073\/pnas.260472897","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:44:19Z","timestamp":1027694659000},"page":"14638-14643","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":133,"title":["Efficient transduction of neural cells\n            <i>in vitro<\/i>\n            and\n            <i>in vivo<\/i>\n            by a baculovirus-derived vector"],"prefix":"10.1073","volume":"97","author":[{"given":"C.","family":"Sarkis","sequence":"first","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"C.","family":"Serguera","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"S.","family":"Petres","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"D.","family":"Buchet","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"J.-L.","family":"Ridet","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"L.","family":"Edelman","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche Scientifique, H\u00f4pital de la\r Piti\u00e9-Salp\u00e9tri\u00eare, B\u00e2timent Centre d'Etudes et\r de Recherche en Virologie et Immunologie, 83, Boulevard de\r l'H\u00f4pital, 75013 Paris, France; and\r D\u00e9partement de Technologies Cellulaires, Institut\r Pasteur 28, Rue du Docteur Roux, 75015 Paris, France"}]},{"given":"J.","family":"Mallet","sequence":"additional","affiliation":[{"name":"Laboratoire de G\u00e9n\u00e9tique Mol\u00e9culaire des\r Processus Neurod\u00e9g\u00e9n\u00e9ratifs et de la\r Neurotransmission, Unit\u00e9 Mixte de Recherche 9923, Centre National\r de la Recherche 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