{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:59:10Z","timestamp":1779343150056,"version":"3.51.4"},"reference-count":26,"publisher":"Proceedings of the National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,8,5]]},"abstract":"<jats:p>\n            Using clathrin-minus\n            <jats:italic>Dictyostelium<\/jats:italic>\n            cells, we identified a novel requirement for clathrin during cytokinesis. In suspension culture, clathrin-minus cells failed to divide and became large and multinucleate. This cytokinesis deficiency was not attributable to a pleiotropic effect on the actomyosin cytoskeleton, since other cellular events driven by myosin II (e.g., cortical contraction and capping of concanavalin A receptors) remained intact in clathrin-minus cells. Examination of cells expressing myosin II tagged with green fluorescent protein showed that clathrin-minus cells failed to assemble myosin II into a functional contractile ring. This inability to localize myosin II to a particular location was specific for cytokinesis, since clathrin-minus cells moving across a substrate localized myosin II properly to their posterior cortexes. These results demonstrate that clathrin is essential for construction of a functional contractile ring during cell division.\n          <\/jats:p>","DOI":"10.1073\/pnas.94.16.8575","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:35:50Z","timestamp":1027694150000},"page":"8575-8578","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":75,"title":["A novel role for clathrin in\u2009cytokinesis"],"prefix":"10.1073","volume":"94","author":[{"given":"Maria L.","family":"Niswonger","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa J.","family":"O\u2019Halloran","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[1997,8,5]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511529764"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.6.1321"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(96)80054-8"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90152-X"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.1.165"},{"key":"e_1_3_3_6_2","first-page":"229a","volume":"7","author":"Field C M","year":"1996","unstructured":"C M Field, K F Oegema, S Doberstein, B M Alberts, T J Mitchison Mol Biol Cell 7, 229a, (abstr.). 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