{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:05:08Z","timestamp":1779343508742,"version":"3.51.4"},"reference-count":49,"publisher":"Proceedings of the National Academy of Sciences","issue":"22","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1997,10,28]]},"abstract":"<jats:p>\n            RNA polymerase I (pol I) is a nuclear enzyme whose function is to transcribe the duplicated genes encoding the precursor of the three largest ribosomal RNAs. We report a cell-free system from broccoli (\n            <jats:italic>Brassica oleracea<\/jats:italic>\n            ) inflorescence that supports promoter-dependent RNA pol I transcription\n            <jats:italic>in vitro<\/jats:italic>\n            . The transcription system was purified extensively by DEAE-Sepharose, Biorex 70, Sephacryl S300, and Mono Q chromatography. Activities required for pre-rRNA transcription copurified with the polymerase on all four columns, suggesting their association as a complex. Purified fractions programmed transcription initiation from the\n            <jats:italic>in vivo<\/jats:italic>\n            start site and utilized the same core promoter sequences required\n            <jats:italic>in vivo<\/jats:italic>\n            . The complex was not dissociated in 800 mM KCl and had a molecular mass of nearly 2 MDa based on gel filtration chromatography. The most highly purified fractions contain \u224830 polypeptides, two of which were identified immunologically as RNA polymerase subunits. These data suggest that the occurrence of a holoenzyme complex is probably not unique to the pol II system but may be a general feature of eukaryotic nuclear polymerases.\n          <\/jats:p>","DOI":"10.1073\/pnas.94.22.11869","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:40:52Z","timestamp":1027694452000},"page":"11869-11874","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":54,"title":["Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription\n            <i>in\u2009vitro<\/i>"],"prefix":"10.1073","volume":"94","author":[{"given":"Julio","family":"Saez-Vasquez","sequence":"first","affiliation":[{"name":"Biology Department, Washington University, Campus Box 1137, One Brookings Drive, St. Louis, MO 63130"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig 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