{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T01:03:07Z","timestamp":1772931787738,"version":"3.50.1"},"reference-count":25,"publisher":"Proceedings of the National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,1,20]]},"abstract":"<jats:p>Blood cell transplantation is largely replacing bone marrow transplantation because engraftment is more rapid. This accelerated engraftment is thought to be mediated by relatively mature committed hematopoietic progenitor cells. Herein, we have used a modified rhodamine (Rho) staining procedure to identify and purify Rho<jats:sup>+\/++<\/jats:sup>(dull\/bright) and Rho<jats:sup>\u2212<\/jats:sup>(negative) subpopulations of hematopoietic progenitor cells in murine cytokine-mobilized blood. The Rho<jats:sup>+\/++<\/jats:sup>cell population contained &gt;99% of committed progenitor cells with<jats:italic>in vitro<\/jats:italic>colony-forming ability. The Rho<jats:sup>\u2212<\/jats:sup>cell population contained the majority of hematopoietic stem cells with<jats:italic>in vivo<\/jats:italic>marrow repopulating ability. The rate of hematopoietic reconstitution was identical in recipients of grafts containing only purified Rho<jats:sup>\u2212<\/jats:sup>stem cells or purified Rho<jats:sup>\u2212<\/jats:sup>stem cells in combination with large numbers of Rho<jats:sup>+\/++<\/jats:sup>committed progenitor cells. In contrast, transplantation of 3-fold more hematopoietic stem cells resulted in accelerated reconstitution, indicating that the reconstitution rate was determined by the absolute numbers of Rho<jats:sup>\u2212<\/jats:sup>stem cells in the graft. In addition, we observed a 5- to 8-fold reduced frequency of the subset of hematopoietic stem cells with long-term repopulating ability in cytokine-mobilized blood in comparison to steady-state bone marrow. Our results indicate that hematopoietic stem cells and not committed progenitor cells mediate early hematopoietic reconstitution after blood cell transplantation and that relative to bone marrow, the frequency of stem cells with long-term repopulating ability is reduced in mobilized blood.<\/jats:p>","DOI":"10.1073\/pnas.95.2.725","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:40:11Z","timestamp":1027694411000},"page":"725-729","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":86,"title":["The early phase of engraftment after murine blood cell transplantation is mediated by hematopoietic\u2009stem\u2009cells"],"prefix":"10.1073","volume":"95","author":[{"given":"J. Mark J. M.","family":"Zijlmans","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Jan W. M.","family":"Visser","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Laurens","family":"Laterveer","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Karin","family":"Kleiverda","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Diana P. M.","family":"Heemskerk","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Philip M.","family":"Kluin","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Roel","family":"Willemze","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]},{"given":"Willem E.","family":"Fibbe","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Hematology, Department of Hematology, and Department of Pathology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands; and Lindsley F. Kimball Research Institute, Laboratory of Stem Cell Biology, New York Blood Center, New York, NY 10021"}]}],"member":"341","published-online":{"date-parts":[[1998,1,20]]},"reference":[{"key":"e_1_3_2_1_2","first-page":"248","volume":"18","author":"Visser J W M","year":"1990","unstructured":"J W M Visser, D W Van Bekkum Exp Hematol 18, 248\u2013256 (1990).","journal-title":"Exp Hematol"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(93)90002-A"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199403243301206"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.2.397.397"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/347188a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.6.1443"},{"key":"e_1_3_2_7_2","first-page":"120","volume":"7","author":"Ploemacher R E","year":"1993","unstructured":"R E Ploemacher, J C M Van der Loo, C A J Van Beurden, M R M Baert Leukemia 7, 120\u2013130 (1993).","journal-title":"Leukemia"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.12.3463.3463"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.1.93"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90711-3"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.6.1905.1905"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)90795-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"2332","DOI":"10.4049\/jimmunol.142.7.2332","volume":"142","author":"Iscove N N","year":"1989","unstructured":"N N Iscove, A R Shaw, G Keller J Immunol 142, 2332\u20132337 (1989).","journal-title":"J Immunol"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.6.1405.bloodjournal8061405"},{"key":"e_1_3_2_15_2","first-page":"689","volume":"14","author":"Juttner C A","year":"1994","unstructured":"C A Juttner, W E Fibbe, J Nemunaitis, L Kanz, A M Gianni Bone Marrow Transplant 14, 689\u2013693 (1994).","journal-title":"Bone Marrow Transplant"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199508033330503"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.19.8901"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90141-K"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2898810"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90037-X"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.2.220"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.12.3758.3758"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10134"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15346"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.10.3596.3596_3596_3606"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.95.2.725","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T12:26:37Z","timestamp":1682166397000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.95.2.725"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1,20]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,1,20]]}},"alternative-id":["10.1073\/pnas.95.2.725"],"URL":"https:\/\/doi.org\/10.1073\/pnas.95.2.725","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1,20]]},"assertion":[{"value":"1997-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1997-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1998-01-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}