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This technique enabled us to examine synthesis of \u03b2-glucan as a fiber with a two-dimensional structure. The synthesis of \u03b2-glucan microfibrils was directed in arrays by cortical microtubules at many loci on the membrane sheets. The microfibrils were mainly arranged along the microtubules, but the distribution of microfibrils was not always parallel to that of the microtubules. The rate of \u03b2-glucan elongation as determined directly on the exoplasmic surface was 620 nm per min. When the assembly of microtubules was disrupted by treatment with propyzamide, the \u03b2-glucans were not deposited in arrays but in masses. This finding shows that the arrayed cortical microtubules are not required for \u03b2-glucan synthesis but are required for the formation of arranged microfibrils on the membrane sheet.<\/jats:p>","DOI":"10.1073\/pnas.95.25.15102","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:11Z","timestamp":1027680011000},"page":"15102-15106","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":28,"title":["<i>In situ<\/i>\n                    synthesis of \u03b2-glucan microfibrils on tobacco plasma membrane sheets"],"prefix":"10.1073","volume":"95","author":[{"given":"Noriko","family":"Hirai","sequence":"first","affiliation":[{"name":"Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611, Japan; and Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo 678-12, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Sonobe","sequence":"additional","affiliation":[{"name":"Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611, Japan; and Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo 678-12, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahisa","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Wood Research Institute, Kyoto University, Gokasho, Uji, Kyoto 611, Japan; and Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo 678-12, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1998,12,8]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.31.060180.000411"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.35.060184.003101"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01314675"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00395959"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60176-X"},{"key":"e_1_3_2_6_2","first-page":"85","volume-title":"The Cytoskeletal Basis of Plant Growth and Form","author":"Giddings T H","year":"1991","unstructured":"T H Giddings, L A Staehelin The Cytoskeletal Basis of Plant Growth and Form, ed C W Lloyd (Academic, London), pp. 85\u201399 (1991)."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.13.6050"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00394482"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01354001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01279847"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00272899"},{"key":"e_1_3_2_12_2","first-page":"1147","volume":"31","author":"Sonobe S","year":"1990","unstructured":"S Sonobe Plant Cell Physiol 31, 1147\u20131153 (1990).","journal-title":"Plant Cell Physiol"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(84)85106-X"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.1985.Supplement_2.3"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9353"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.61.1500"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.61.6.938"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.66.5.911"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/35.9.1342"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.83.4.1054"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.101.4.1131"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00391181"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.82.1.301"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.14.7679"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01285036"},{"key":"e_1_3_2_26_2","first-page":"451","volume":"35","author":"Sonobe S","year":"1994","unstructured":"S Sonobe, S Takahashi Plant Cell Physiol 35, 451\u2013460 (1994).","journal-title":"Plant Cell Physiol"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.22.12637"},{"key":"e_1_3_2_28_2","first-page":"1","volume":"84","author":"Ihara Y","year":"1997","unstructured":"Y Ihara, F Sakai, T Hayashi Wood Res 84, 1\u20136 (1997).","journal-title":"Wood Res"},{"key":"e_1_3_2_29_2","unstructured":"Kawagoe Y. & Delmer D. 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