{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:26:49Z","timestamp":1781101609749,"version":"3.54.1"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1998,3,31]]},"abstract":"<jats:p>\n                    We postulate a novel and general mechanism in which the redox-active sulfur donor group of cyst(e)ine confers oxidoreductive characteristics on stable zinc sites in proteins. Thus, the present, an earlier, and accompanying manuscripts [Maret, W., Larsen, K. S. &amp; Vallee, B. L. (1997)\n                    <jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic>\n                    94, 2233\u20132237; Jiang, L.-J., Maret, W. &amp; Vallee, B. L. (1998)\n                    <jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic>\n                    95, 3483\u20133488; and Jacob, C., Maret, W. &amp; Vallee, B. L. (1998)\n                    <jats:italic>Proc. Natl. Acad. Sci. USA<\/jats:italic>\n                    95, 3489\u20133494] demonstrate that the interactive network featuring multiple zinc\/sulfur bonds as found in the clusters of metallothionein (MT) constitutes a coordination unit critical for the concurrent oxidation of cysteine ligands and the ensuing release of zinc. The low position of MT (&lt;\u2212366 mV) on a scale of redox reagents allows its effective oxidation by relatively mild cellular oxidants, in particular disulfides. When MT is exposed to an excess of dithiodipyridine, all of its 20 cysteines are oxidized within 1 hr with the concomitant release of all 7 zinc atoms; similarly, the thiol\/disulfide oxidoreductase DsbA reacts stoichiometrically with MT to release zinc. Zinc and sulfur ligands in the clusters are in a spatial arrangement that seemingly favors disulfide bond formation. Jointly, this and the above-mentioned manuscripts conclude that the control of cellular zinc distribution as a function of the energy state of the cell is the long sought role of MT. This specific MT function renders dubious the widely held belief that MT primarily scavenges radicals or detoxifies metals and is consistent with the frequent use of cysteine as a zinc ligand in proteins as a means of both tight\n                    <jats:italic>and<\/jats:italic>\n                    weak zinc binding of thiols and disulfides, respectively. Thus, we relate changes in the reducing power of the cell to the stability of the zinc\/sulfur network in MT and the relative mobility of zinc and its control.\n                  <\/jats:p>","DOI":"10.1073\/pnas.95.7.3478","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:40:11Z","timestamp":1027680011000},"page":"3478-3482","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":487,"title":["Thiolate ligands in metallothionein confer redox activity on zinc clusters"],"prefix":"10.1073","volume":"95","author":[{"given":"Wolfgang","family":"Maret","sequence":"first","affiliation":[{"name":"Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Seeley G. 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