{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:58:58Z","timestamp":1767859138003,"version":"3.49.0"},"reference-count":30,"publisher":"Proceedings of the National Academy of Sciences","issue":"2","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,1,16]]},"abstract":"<jats:p>\n            CpG islands are important in the protection of adjacent\n housekeeping genes from\n            <jats:italic>de novo<\/jats:italic>\n            DNA methylation and for\n keeping them in a transcriptionally active state. However, little is\n known about their capacity to protect heterologous genes and assure\n position-independent transcription of adjacent transgenes or retroviral\n vectors. To tackle this question, we have used the mouse\n            <jats:italic>aprt<\/jats:italic>\n            CpG island to flank a Rous sarcoma virus\n (RSV)-derived reporter vector and followed the transcriptional activity\n of integrated vectors. RSV is an avian retrovirus which does not\n replicate in mammalian cells because of several blocks at all levels of\n the replication cycle. Here we show that our RSV-derived reporter\n proviruses linked to the mouse\n            <jats:italic>aprt<\/jats:italic>\n            gene CpG island\n remain undermethylated and keep their transcriptional activity after\n stable transfection into both avian and nonpermissive mammalian cells.\n This effect is most likely caused by the protection from\n            <jats:italic>de\n novo<\/jats:italic>\n            methylation provided by the CpG island and not by\n enhancement of the promoter strength. Our results are consistent with\n previous finding of CpG islands in proximity to active but not inactive\n proviruses and support further investigation of the protection of the\n gene transfer vectors from DNA methylation.\n          <\/jats:p>","DOI":"10.1073\/pnas.98.2.565","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T14:45:09Z","timestamp":1027694709000},"page":"565-569","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":47,"title":["CpG island protects Rous sarcoma virus-derived vectors integrated into nonpermissive cells from DNA methylation and transcriptional suppression"],"prefix":"10.1073","volume":"98","author":[{"given":"Jir\u0306\u00ed","family":"Hejnar","sequence":"first","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"H\u00e1jkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jir\u0306\u00ed","family":"Plach\u00fd","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Elleder","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volodymyr","family":"Stepanets","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Svoboda","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, Academy of Sciences of the Czech\r Republic, Flemingovo n\u00e1m. 2, CZ 16637 Prague 6, Czech\r Republic; and Max-Planck-Institut of Molecular\r Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"341","published-online":{"date-parts":[[2001,1,16]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/321209a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)89009-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.19.2282"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/371435a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00571-4"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(74)90042-7"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1974.039.01.113"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04271.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(82)90137-4"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.13.5193"},{"key":"e_1_3_3_11_2","first-page":"277","volume":"5","author":"Hejnar J","year":"1994","unstructured":"J Hejnar, J Svoboda, J Geryk, V J Fincham, R H\u00e1k Cell Growth Differ 5, 277\u2013285 (1994).","journal-title":"Cell Growth Differ"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3006249"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9597"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80951-1"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00200-4"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.1.461-463.1991"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000149682"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.10.4679-4687.2000"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160159597"},{"key":"e_1_3_3_20_2","first-page":"3720","volume":"74","author":"Agarval M","year":"2000","unstructured":"M Agarval, T W Austin, F Morel, J Chen, E Bohnlein, I Plavec J Virol 74, 3720\u20133728 (2000).","journal-title":"J Virol"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.6.2671-2678.2000"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.21.8869"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.9.1513"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.24.5064"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54187-9"},{"key":"e_1_3_3_26_2","first-page":"309","volume":"5","author":"Svoboda J","year":"1992","unstructured":"J Svoboda, J Plach\u00fd, J Hejnar, I Karakoz, R V Guntaka, J Geryk Immunogenetics 5, 309\u2013315 (1992).","journal-title":"Immunogenetics"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(97)01181-5"},{"key":"e_1_3_3_28_2","first-page":"561","volume-title":"Epigenetic Mechanisms of Gene Regulation","author":"Nyce J W","year":"1996","unstructured":"J W Nyce Epigenetic Mechanisms of Gene Regulation, eds V E Russo, R A Martienssen, A D Riggs (Cold Spring Harbor Lab. 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