{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:45:28Z","timestamp":1791341128281,"version":"4.3.1"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"1","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,1,8]]},"abstract":"<jats:p>\n                    Over the past decade xenotransplantation systems have been used with increasing success to gain a better understanding of human cells that are able to initiate and maintain the hematopoietic system\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The nonobese diabetic\/severe combined immunodeficiency (SCID) mouse has been a particularly useful model. Human cells capable of hematopoietic repopulation in this mouse, termed SCID-repopulating cells, have been assumed to represent the most primitive elements of the hematopoietic system, responsible for long-term maintenance of hematopoiesis. However, we demonstrate that SCID-repopulating cells present in the CD34\n                    <jats:sup>+<\/jats:sup>\n                    cell fraction of cord blood can be segregated into subpopulations with distinct repopulation characteristics. CD34\n                    <jats:sup>+<\/jats:sup>\n                    \/CD38\n                    <jats:sup>+<\/jats:sup>\n                    progenitors can repopulate recipients rapidly, but can only maintain the graft for 12 weeks or less and have no secondary repopulation potential. Conversely, the more primitive CD34\n                    <jats:sup>+<\/jats:sup>\n                    \/CD38\n                    <jats:sup>\u2212<\/jats:sup>\n                    subpopulation repopulates recipients more gradually, can maintain the graft for at least 20 weeks, and contains cells with serial repopulation potential throughout the engraftment period. Additionally, a much higher frequency of T cell precursors are found among SCID-repopulating cells in the CD34\n                    <jats:sup>+<\/jats:sup>\n                    \/CD38\n                    <jats:sup>\u2212<\/jats:sup>\n                    subpopulation. These findings demonstrate that cells with variable repopulation potential comprise the human CD34\n                    <jats:sup>+<\/jats:sup>\n                    population and that short- and long-term potential of human precursors can be evaluated in the mouse model.\n                  <\/jats:p>","DOI":"10.1073\/pnas.012336799","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:36:44Z","timestamp":1027679804000},"page":"413-418","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":141,"title":["Differential long-term and multilineage engraftment potential from subfractions of human CD34\n                    <sup>+<\/sup>\n                    cord blood cells transplanted into NOD\/SCID mice"],"prefix":"10.1073","volume":"99","author":[{"given":"Christopher J.","family":"Hogan","sequence":"first","affiliation":[{"name":"Bone Marrow Transplant Program, Division of Medical Oncology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262; and Institute for Gene Therapy and Molecular Medicine, The Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth J.","family":"Shpall","sequence":"additional","affiliation":[{"name":"Bone Marrow Transplant Program, Division of Medical Oncology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262; and Institute for Gene Therapy and Molecular Medicine, The Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Keller","sequence":"additional","affiliation":[{"name":"Bone Marrow Transplant Program, Division of Medical Oncology, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, CO 80262; and Institute for Gene Therapy and Molecular Medicine, The Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,1,8]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(92)90002-V"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.11.110195.000343"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/347188a0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.2.487.bloodjournal882487"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.2.220"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.171.5.1407"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2788"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02737.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.1.93"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(94)90037-X"},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.4049\/jimmunol.154.1.180","volume":"154","author":"Shultz L D","year":"1995","unstructured":"L D Shultz, P A Schweitzer, S W Christianson, B Gott, I B Schweitzer, B Tennent, S McKenna, L Mobraaten, T V Rajan, D L Greiner, et al. 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